Grand Challenge in Membrane Biology
EMSL Project ID
14398
Abstract
In this grand challenge, we will use a systems approach to understand the network of genes and proteins that govern the structure and function of membranes and their components responsible for photosynthesis and nitrogen fixation in cyanobacteria (blue-green algae). A systems approach integrates all temporal information into a predictive, dynamic model to understand the function of a cell and the cellular membranes. These microorganisms make significant contributions to harvesting solar energy, planetary carbon sequestration, metal acquisition, and hydrogen production in marine and freshwater ecosystems. Cyanobacteria are also model microorganisms for studying the fixation of carbon dioxide through photosynthesis at the biomolecular level. The results of this Grand Challenge will provide the first comprehensive systems level understanding of how environmental conditions influence key carbon fixation processes at the gene-protein-organism level. Specific tasks associated with this grand challenge will be submitted individually.
Project Details
Project type
Grand Challenge
Start Date
2005-03-31
End Date
2011-04-03
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Buchko GW (2009) "Pentapeptide Repeat Proteins and Cyanobacteria." In Handbook on Cyanobacteria: Biochemistry, Biotechnology and Applications, pp. 233-257. Nova Science Publishers, Hauppauge, NY.
Buchko GW, and HJ Sofia. 2008. "Backbone 1H, 13C, and 15N NMR assignments for the Cyanothece 51142 protein cce_0567: a protein associated with nitrogen fixation in the DUF683 family." Biomolecular NMR Assignments 2:25-28.
Buchko GW, and H Robinson. 2012. "Crystal structure of cce_0566 from Cyanothece 51142, a protein associated with nitrogen fixation in the DUF269 family." FEBS Letters 586(4):350-355. doi:10.1016/j.febslet.2012.01.037
Buchko GW, H Robinson, and A Addlagatta. 2009. "Structural characterization of the protein cce_0567 from Cyanothece 51142, a metalloprotein associated with nitrogen fixation in the DUF683 family." Biochimica et Biophysica Acta--Proteins and Proteomics 1794(4):627-633.
Buchko GW, H Robinson, HB Pakrasi, and MA Kennedy. 2008. "Insights into the structural variation between pentapeptide repeat proteins - Crystal structure of Rfr23 from Cyanothece 51142." Journal of Structural Biology 162(1):184-192.
Buchko GW, H Robinson, S Ni, HB Pakrasi, and MA Kennedy. 2006. "Cloning, expression, crystallization and preliminary crystallographic analysis of a pentapeptide-repeat protein (Rfr23) from bacterium Cyanothece 511421." Acta Crystallographica. Section F 62(12):1251-1254. doi:10.1107/S174430910604663X
Buchko GW, S Ni, H Robinson, EA Welsh, HB Pakrasi, and MA Kennedy. 2006. "Characterization of two potentially universal turn motifs that shape the repeated five-residues fold - Crystal structure of a lumenal pentapeptide repeat protein from Cyanothece 51142." Protein Science 15(11):2579-2595
Fukuzawa H, T Ogawa, and A Kaplan. 2011. "The Uptake of CO2 by Cyanobacteria and Microalgae." Chapter 25 in Photosynthesis Plastid Biology, Energy Conversion and Carbon Assimilation, vol. 34, ed. Julian J. Eaton-Rye, Baishnab C. Tripathy, Thomas D. Sharkey, pp. 625-650. Springer Dordrecht Heidelberg London New York , New York .
in press. Fukuzawa, H., Ogawa, T. and Kaplan, A. (2009) The Uptake of CO2 by Cyanobacteria and Microalgae. In: PHOTOSYNTHESIS: A Comprehensive Treatise of Physiology, Biochemistry, Biophysics and Molecular Biology Edited by Eaton-Rye, J. and Tripathy, B. Springer-Verlag, Berlin..
Kaplan A, M Hagemann, H Bauwe, S Kahlon, and T Ogawa. 2008. "Carbon acquisition by Cyanobacteria: Mechanisms, Comparative Genomics and Evolution." Chapter 12 in The Cyanobacteria: Molecular Biology, Genomics and Evolution, ed. Antonia Herrero; Enrique Flores. Caister Academic Press, Norfolk, United Kingdom.
Liberton ML, JR Austin, RH Berg, and HB Pakrasi. 2011. "Unique Thylakoid Membrane Architecture of a Unicellular N2-Fixing Cyanobacterium Revealed by Electron Tomography." Plant Physiology 155(4):1656-1666. doi:10.1104/pp.110.165332
Min H, and LA Sherman. 2010. "Genetic Transformation and Mutagenesis Via Single-Stranded DNA in the Unicellular, Diazotrophic Cyanobacteria of the Genus Cyanothece." Applied and Environmental Microbiology 76(22):7641-7645. doi:10.1128/AEM.01456-10.
Ogawa T, and H Mi. 2007. "Cyanobacterial NADPH dehydrogenase complexes." Photosynthesis Research 93(1-3):69-77. doi:10.1007/s11120-006-9128-y
Stockel J, EA Welsh, ML Liberton, R Kunnavakkam, R Aurora, and HB Pakrasi. 2008. "Global transcriptomic analysis of Cyanothece 51142 reveals robust diurnal oscillation of central metabolic processes ." Proceedings of the National Academy of Sciences of the United States of America 105(16):6156-6161. doi:10.1073/pnas.0711068105
Summerfield T, J Toepel, and LA Sherman. 2008. "Low-Oxygen Induction of Normally Cryptic psbA Genes in Cyanobacteria." Biochemistry 47(49):12939–12941. doi:10.1021/bi8018916
Toepel J, EA Welsh, T Summerfield, HB Pakrasi, and LA Sherman. 2008. "Differential Transcriptional Analysis of the Cyanobacterium Cyanothece sp. Strain ATCC 51142 during Light-Dark and Continuous-Light Growth." Journal of Bacteriology 190(11):3904-3913. doi:10.1128/JB.00206-08
Toepel J, JE McDermott, T Summerfield, and LA Sherman. 2009. "Transcriptional analysis of the unicellular, diazotrophic cyanobacterium Cyanothece sp. ATCC 51142 grown under short day/night cycles." Journal of Phycology 45(3):610-620.
Wegener KM, EA Welsh, LE Thornton, NS Keren, JM Jacobs, KK Hixson, ME Monroe, DG Camp, II, RD Smith, and HB Pakrasi. 2008. "High Sensitivity Proteomics Assisted Discovery of a Novel Operon Involved in the Assembly of Photosystem II, a Membrane Protein Complex." Journal of Biological Chemistry 283(41):27829-27837. doi:10.1074/jbc.M803918200
Welsh EA, ML Liberton, J Stockel, T Loh, TR Elvitigala, C Wang, A Wollam, RS Fulton, SW Clifton, JM Jacobs, R Aurora, BK Ghosh, LA Sherman, RD Smith, RK Wilson, and HB Pakrasi. 2008. "The genome of Cyanothece 51142, a unicellular diazotrophic cyanobacterium important in the marine nitrogen cycle." Proceedings of the National Academy of Sciences of the United States of America 105(39):15094-15099. doi:10.1073/pnas.0805418105
Xu M, G Bernat, AK Singh, H Mi, M Rogner, HB Pakrasi, and T Ogawa. 2008. "Properties of Mutants of Synechocystis sp. Strain PCC 6803 Lacking Inorganic Carbon Sequestration Systems." Plant & Cell Physiology 49(11):1672-1677. doi:10.1093/pcp/pcn139
Xu M, T Ogawa, HB Pakrasi, and H Mi. 2008. "Identification and Localization of the CupB Protein Involved in Constitutive CO? uptake in the Cyanobacterium, Synechocystis sp. Strain PCC 6803." Plant & Cell Physiology 49(6):994-997. doi:10.1093/pcp/pcn074