Grand Challenge in Membrane Biology
EMSL Project ID
14398
Abstract
In this grand challenge, we will use a systems approach to understand the network of genes and proteins that govern the structure and function of membranes and their components responsible for photosynthesis and nitrogen fixation in cyanobacteria (blue-green algae). A systems approach integrates all temporal information into a predictive, dynamic model to understand the function of a cell and the cellular membranes. These microorganisms make significant contributions to harvesting solar energy, planetary carbon sequestration, metal acquisition, and hydrogen production in marine and freshwater ecosystems. Cyanobacteria are also model microorganisms for studying the fixation of carbon dioxide through photosynthesis at the biomolecular level. The results of this Grand Challenge will provide the first comprehensive systems level understanding of how environmental conditions influence key carbon fixation processes at the gene-protein-organism level. Specific tasks associated with this grand challenge will be submitted individually.
Project Details
Project type
Grand Challenge
Start Date
2005-03-31
End Date
2011-04-03
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Crystal structure of cce_0566 fromCyanothece 51142, a protein associated with nitrogen fixation in the DUF269 family
Buchko GW, and H Robinson. 2012. "Crystal structure of cce_0566 from Cyanothece 51142, a protein associated with nitrogen fixation in the DUF269 family." FEBS Letters 586(4):350-355. doi:10.1016/j.febslet.2012.01.037
Cyanobacterial NADPH dehydrogenase complexes
Ogawa T, and H Mi. 2007. "Cyanobacterial NADPH dehydrogenase complexes." Photosynthesis Research 93(1-3):69-77. doi:10.1007/s11120-006-9128-y
Unique Thylakoid Membrane Architecture of a Unicellular N2-Fixing Cyanobacterium Revealed by Electron Tomography
Liberton ML, JR Austin, RH Berg, and HB Pakrasi. 2011. "Unique Thylakoid Membrane Architecture of a Unicellular N2-Fixing Cyanobacterium Revealed by Electron Tomography." Plant Physiology 155(4):1656-1666. doi:10.1104/pp.110.165332
Genetic Transformation and Mutagenesis via Single-Stranded DNA in the Unicellular, Diazotrophic Cyanobacteria of the Genus Cyanothece
Min H, and LA Sherman. 2010. "Genetic Transformation and Mutagenesis Via Single-Stranded DNA in the Unicellular, Diazotrophic Cyanobacteria of the Genus Cyanothece." Applied and Environmental Microbiology 76(22):7641-7645. doi:10.1128/AEM.01456-10.
Properties of Mutants of Synechocystis sp. Strain PCC 6803 Lacking Inorganic Carbon Sequestration Systems
Xu M, G Bernat, AK Singh, H Mi, M Rogner, HB Pakrasi, and T Ogawa. 2008. "Properties of Mutants of Synechocystis sp. Strain PCC 6803 Lacking Inorganic Carbon Sequestration Systems." Plant & Cell Physiology 49(11):1672-1677. doi:10.1093/pcp/pcn139
Low-Oxygen Induction of Normally Cryptic psbA Genes in Cyanobacteria
Summerfield T, J Toepel, and LA Sherman. 2008. "Low-Oxygen Induction of Normally Cryptic psbA Genes in Cyanobacteria." Biochemistry 47(49):12939–12941. doi:10.1021/bi8018916
Differential Transcriptional Analysis of the Cyanobacterium Cyanothece sp. Strain ATCC 51142 during Light-Dark and Continuous-Light Growth
Toepel J, EA Welsh, T Summerfield, HB Pakrasi, and LA Sherman. 2008. "Differential Transcriptional Analysis of the Cyanobacterium Cyanothece sp. Strain ATCC 51142 during Light-Dark and Continuous-Light Growth." Journal of Bacteriology 190(11):3904-3913. doi:10.1128/JB.00206-08
Global transcriptomic analysis of Cyanothece 51142 reveals robust diurnal oscillation of central metabolic processes
Stockel J, EA Welsh, ML Liberton, R Kunnavakkam, R Aurora, and HB Pakrasi. 2008. "Global transcriptomic analysis of Cyanothece 51142 reveals robust diurnal oscillation of central metabolic processes ." Proceedings of the National Academy of Sciences of the United States of America 105(16):6156-6161. doi:10.1073/pnas.0711068105
The genome of Cyanothece 51142, a unicellular diazotrophic cyanobacterium important in the marine nitrogen cycle
Welsh EA, ML Liberton, J Stockel, T Loh, TR Elvitigala, C Wang, A Wollam, RS Fulton, SW Clifton, JM Jacobs, R Aurora, BK Ghosh, LA Sherman, RD Smith, RK Wilson, and HB Pakrasi. 2008. "The genome of Cyanothece 51142, a unicellular diazotrophic cyanobacterium important in the marine nitrogen cycle." Proceedings of the National Academy of Sciences of the United States of America 105(39):15094-15099. doi:10.1073/pnas.0805418105
High Sensitivity Proteomics Assisted Discovery of a Novel Operon Involved in the Assembly of Photosystem II, a Membrane Protein Complex
Wegener KM, EA Welsh, LE Thornton, NS Keren, JM Jacobs, KK Hixson, ME Monroe, DG Camp, II, RD Smith, and HB Pakrasi. 2008. "High Sensitivity Proteomics Assisted Discovery of a Novel Operon Involved in the Assembly of Photosystem II, a Membrane Protein Complex." Journal of Biological Chemistry 283(41):27829-27837. doi:10.1074/jbc.M803918200
Identification and Localization of the CupB Protein Involved in Constitutive CO2 Uptake in the Cyanobacterium, Synechocystis sp. Strain PCC 6803
Xu M, T Ogawa, HB Pakrasi, and H Mi. 2008. "Identification and Localization of the CupB Protein Involved in Constitutive CO? uptake in the Cyanobacterium, Synechocystis sp. Strain PCC 6803." Plant & Cell Physiology 49(6):994-997. doi:10.1093/pcp/pcn074
Cloning, expression, crystallization and preliminary crystallographic analysis of a pentapeptide-repeat protein (Rfr23) from the bacteriumCyanothece 51142
Buchko GW, H Robinson, S Ni, HB Pakrasi, and MA Kennedy. 2006. "Cloning, expression, crystallization and preliminary crystallographic analysis of a pentapeptide-repeat protein (Rfr23) from bacterium Cyanothece 511421." Acta Crystallographica. Section F 62(12):1251-1254. doi:10.1107/S174430910604663X