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Identify Biomarkers for COPD (Chronic Obstructive Pulmonary Disease) in Humans using Proteomic and Metabonomic Analysis of Serum and Urine.


EMSL Project ID
14597

Abstract

Chronic Obstructive Pulmonary Disease (COPD) is a slowly progressive disease of the airways that is characterized by a gradual loss of lung function. COPD is a major cause of death and illness throughout the world, and is the fourth leading cause of death in the United States. The objective of this study is to identify putative biomarkers that may be predictive for, or correlate with, progression of COPD. Ultimately, and after extensive validation, the biomarkers may be useful for diagnosis and the determination of molecular targets and pathways. To accomplish this objective, 40 de-identified blood plasma samples from COPD patients at differing stages of COPD will be provided by the University of Utah through LineaGen Research Corporation (formerly GenData). Proteomic analysis of these samples to determine the proteins present at differing stages of COPD will be accomplished using Fourier transform ion cyclotron resonance (FTICR) mass spectrometry. Additionally, we will analyze a small number of samples by 'gel-free' 2-D capillary liquid chromatography (LC)-MS/MS to extend the human plasma PMT database to include peptides from subjects with COPD.

Project Details

Start Date
2005-05-16
End Date
2008-05-18
Status
Closed

Team

Principal Investigator

Joel Pounds
Institution
Pacific Northwest National Laboratory

Team Members

Susan Varnum
Institution
Pacific Northwest National Laboratory

Related Publications

Rana GSJB, TP York, JS Edmiston, BK Zedler, JG Pounds, JN Adkins, RD Smith, Z Liu, G Li, BT Webb, EL Murrelle, and JW Flora. 2010. "Proteomic biomarkers in plasma that differentiate rapid and slow decline in lung function in adult cigarette smokers with chronic obstructive pulmonary disease (COPD) ." Analytical and Bioanalytical Chemistry 397:1809-1819. doi:10.1007/s00216-010-3742-4