PNNL Metabolomics Measurement and Validation Development for Renewable Energy Research
EMSL Project ID
16092b
Abstract
This capability-development project will provide PNNL and DOE with a unique live / in-situ metabolomics capability for microbial bioenergy development. The technology will be applicable for the development of bio-based products including ethanol, antimicrobial agents and amino acids. Deliverables include: (1) Novel metabolomics capabilities for prokaryotic cultures under controlled-growth conditions. (2) Compatibility with existing genomics and proteomics procedures. (3) A supporting technology for current and future PNNL systems biology research. (4) A detailed characterization of C1, C2 and C6 metabolism in suspended Thermotoga lettingae samples under electron acceptor limited conditions as a first demonstration of bioreactor capabilities. (5) The serial metabolomics paradigm and supporting live/in-situ technologies may constitute patentable intellectual property. (6) Help to fulfill DOE OBER's investment plan of Advancing Science and Technology for Bioenergy Solutions, to conduct comprehensive, multidisciplinary research programs focused on microbes and plants to drive scientific breakthroughs necessary for the development of cost effective biofuels and bioenergy production and enable the design of microbes that address critical energy or waste cleanup needs (http://www.science.doe.gov/News_Information/News_Room/2007/Budget/SC-DOE_DrOrbach_FY08BudgetTestimony_March72007-fin.pdf).
Project Details
Project type
Large-Scale EMSL Research
Start Date
2007-05-23
End Date
2008-09-19
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Majors PD, JS McLean and JCM Scholten (2008). " NMR bioreactor development for live in-situ microbial functional analysis," Journal of Magnetic Resonance 192(1): 159-166, doi:10.1016/j.jmr.2008.02.014.