Host Cell Interactions with Human Infectious Diseases, Straub
EMSL Project ID
16297a
Abstract
Pathogenesis is a complex biological process between the human host and the infectious disease agent. In our previous successful collaboration with EMSL scientists (EMSL Project 16297), which lead to a high impact publication in CDCs journal Emerging and Infectious Diseases, we began to unlock the mechanisms of the poorly understood gastrointestinal pathogen Norovirus. Unique 3-D cell culture systems developed by EMSL users allowed, for the first time, the ability to cultivate this pathogen in vitro. TEM imaging revealed that the interaction of the norovirus capsid proteins with their host cells caused significant internal eukaryotic cell membrane rearrangement. Proteomic analysis revealed unique, but as yet, uncharacterized host secreted peptides in response to norovirus challenge. This proposal will seek to understand the biological interactions of viral and host proteins. Understanding these processes will enhance our understanding of infectious diseases and lead to unique opportunities for development of rapid diagnostics, vaccines and therapeutics.
Project Details
Project type
Large-Scale EMSL Research
Start Date
2007-05-29
End Date
2010-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Straub TM, Bartholomew RA, Valdez CO, Valentine NB, Dohnalkova A, Ozanich RM, Bruckner-Lea CJ, Call DR. 2010. Human norovirus infection of Caco-2 cells grown as a three dimensional tissue structure. Journal of Water and Health. in press. doi: 10.2166/wh.2010.106
Straub TM, Honer zu Bentrup K, Orosz-Coghlan P, Dohnalkova A, Mayer BK, Bartholomew RA, Valdez CO, Bruckner-Lea CJ, Gerba CP, Abbaszadegan M, Nickerson CA. 2007. In vitro cell culture infectivity assay for human noroviruses. Emerg Infect Dis. 13(3) 396-403.
Straub TM, RA Bartholomew, CO Valdez, NB Valentine, A Dohnalkova, RM Ozanich, CJ Bruckner-Lea, and DR Call. 2011. "Human Norovirus Infection of Caco-2 Cells Grown as a Three-Dimensional Tissue Structure." Journal of Water and Health 9(2):225-240.