Proteomics of in stiu Biostimulated Sediments
EMSL Project ID
16309
Abstract
The proteomics facility will be used for the detection of peptides/proteins belonging to metal-reducing bacteria in sediment samples from naturally-occurring microbial communities. The bacteria within these sediment samples have been subjected to nutrient amendment versus a background sample that did not receive amendment in a effort to stimulate bioreduction of metals in natural microbial communities. As well, we expect to observe an increase in the populations of the requisite bacteria and will therefore attempt to use the AMT tag relative abundances to determine the changes in protein abundance. The appearance/disappearance as well as the increase/decrease in AMT tag, and thus protein, abundances will be compared to existing molecular and physiological datasets in order to determine the changes in population. This will require the inclusion of the existing AMT tag databases from Geobacter sulfurereducens, Geobacter metallireducens, Desulfovibrio desulfuricans G20, and Desulfovibrio vulgaris into a multi-organismal database. Once this database is constructed the peptides stemming from the sediment samples will be analyzed on the LTQ-FT (up to 5 runs per sample) and peak matched against this multi-organismal database.
Project Details
Project type
Exploratory Research
Start Date
2005-10-16
End Date
2007-10-01
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Callister SJ, MJ Wilkins, CD Nicora, KH Williams, JF Banfield, N VerBerkmoes, RL Hettich, AL N'Guessan, P Mouser, H Elifantz, RD Smith, DR Lovley, MS Lipton, and PE Long. 2010. "Analysis of Biostimulated Microbial Communities from Two Field Experiments Reveals Temporal and Spatial Differences in Proteome Profiles." Environmental Science & Technology 44(23):8897-8903. doi:10.1021/es101029f