Analysis of the Proteome and Transcriptome of In Vivo Salmonella typhimurium Infected Ileum
EMSL Project ID
16313
Abstract
The host responses to Salmonella and the Salmonella responses to the host play major roles in the outcome of infection. The present study was aimed to further characterize the molecular pathogenesis of Salmonella enterica Typhimurium infection of the ileal Peyer's patch of neonatal calves as a model for human salmonellosis. Four, two week-old male Holstein calves will undergo laparotomy and loops are prepared in the ileum. The loops are either inoculated with a pathogenic S. typhimurium strain or injected with sterile LB broth as control. Samples for histology, bacteriology, transmission and scanning electron microscopy, RNA extraction for Massively Parallel Signature Sequencing (MPSS) and bovine and Salmonella microarray analyses as well as preparing tryptic digests for proteomic analysis are collected at 5, 15, and 30 minutes and 1, 2, 4 8 and 12 hours post-infection. Fusion and analysis of data from the transcriptome and the proteome for each calf at each time point are expected to provide novel insights into the fundamental biology of infection as new rationales for improved methods to diagnose and prevent salmonellosis in people and animals.
Project Details
Project type
Exploratory Research
Start Date
2005-12-12
End Date
2007-06-28
Status
Closed
Released Data Link
Team
Principal Investigator