Analysis of the Proteome and Transcriptome of In Vivo Brucella spp., Yersinia pestis, Francisella tularensis, Mycobacterium spp., Salmonella typhimurium Infected Ileum
EMSL Project ID
16313a
Abstract
The host responses to Brucella spp., Yersinia pestis, Francisella tularensis, Mycobacterium spp., Salmonella typhimurium and the pathogen responses to the host play major roles in the outcome of infection. The present study is aimed to further characterize the molecular pathogenesis of Brucella spp., Yersinia pestis, Francisella tularensis, Mycobacterium spp., Salmonella typhimurium infection of the ileal Peyer's patch of neonatal calves as a model for human brucellosis, plague, tularemia, Crohn's Disease and salmonellosis. Twenty, three week-old male Holstein calves will undergo laparotomy and loops are prepared in the ileum. The loops are either inoculated with a pathogenic Brucella spp., Yersinia pestis, Francisella tularensis, Mycobacterium spp., Salmonella typhimurium strain or injected with sterile LB broth as control. Samples for histology, bacteriology, transmission and scanning electron microscopy, RNA extraction for gene expression profiling by microarray analysis of bovine and Brucella spp., Yersinia pestis, Francisella tularensis, Mycobacterium spp., Salmonella typhimurium microarray analyses as well as preparing tryptic digests for proteomic analysis are collected at 5, 15, and 30 minutes and 1, 2, 4 8 and 12 hours post-infection. Fusion and analysis of data from the transcriptome and the proteome for each calf at each time point are expected to provide novel insights into the fundamental biology of infection as new rationales for improved methods to diagnose and prevent brucellosis, plague, tularemia, Crohn's Disease and salmonellosis in people and/or animals.
Project Details
Project type
Large-Scale EMSL Research
Start Date
2007-07-09
End Date
2008-07-13
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members