Regulation of Cell Surface Ligand Dynamics (Orr LDRD-BSI, PNNL Scope #90001)
EMSL Project ID
17692
Abstract
We will develop a set of new imaging technologies that can directly visualize ligand oligomerization as well as interaction with other cell surface molecules. We expect to observe an increase in ligand-receptor FRET following stressor/IGF treatment in the case of juxtacrine ligands that are mediating the cellular responses. Alternately, an increase in the rate of release of a given ligand will be characterized by a decrease in ligand-ligand FRET. A very useful aspect of the FRET assay using TIRF microscopy is that it will reveal the specific site of ligand activation in living cells.
Project Details
Project type
Exploratory Research
Start Date
2006-01-19
End Date
2007-07-03
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Harms G., Orr G., Lu H.P (2004) Probing Single-Molecule Ion Channel Conformational Dynamics Using Combined Ultrafast Spectroscopy and Patch-Clamp Recording. Applied Physics Letters. 84:1792-1794.
Harms G.-, Orr G-., Montal M., Thrall B.D., Colson S.D., Lu H.P. (2003) Probing Conformational Changes of Gramicidin Ion Channels by Single-Molecule Patch-Clamp Fluorescence Microscopy. Biophysical Journal. 85:1826-1838.
Oehmen CS, Straatsma TP, Anderson G, Orr G, Webb-Robertson BJ, Taylor R, Mooney R, Baxter B, Jones DR, Dixon DA. (2006). New Challenges Facing Integrative Biological Science in the Post-Genomic Era. Journal of Biological Systems. 14(2)275-293.
Shankaran H, DL Ippolito, WB Chrisler, H Resat, N Bollinger, LK Opresko, and HS Wiley. 2009. "Rapid and Sustained Nuclear-Cytoplasmic ERK Oscillations Induced by Epidermal Growth Factor." Molecular Systems Biology 5:Article Number: 332. doi:10.1038/msb.2009.90