Development of High Throughput Proteomic Production Operations (PNNL Scope # 40601, Dick Smith's OBER Proteomics)
EMSL Project ID
17797
Abstract
This project is developing the capability for high throughput and quantitative measurements of microbial proteomes along with the informatics capabilities necessary to support such applications. Some efforts currently in progress aim to: significantly increase overall data quality of global proteome measurements and provide data that are quantitative and have statistically sound measures of quality; increase overall data production by more than an order of magnitude; provide informatics tools and infrastructure required to support improved data quality and increased throughput and to efficiently manage, use and disseminate large quantities of data generated by GtL "users"; develop foundation for further extension of proteomics measurements to enable more comprehensive coverage of protein modifications. ASSUMPTIONS: The DOE client (OBER) expects us to conduct fundamental research to further our knowledge in areas of importance to DOEs mission, and to disseminate this knowledge through publications in peer-reviewed journals and presentations of research at scientific meetings
Project Details
Start Date
2006-02-02
End Date
2009-02-08
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Baker ES, EA Livesay, DJ Orton, RJ Moore, WF Danielson, DC Prior, YM Ibrahim, BL Lamarche, AM Mayampurath, AA Schepmoes, DF Hopkins, K Tang, RD Smith, and ME Belov. 2010. "An LC-IMS-MS Platform Providing Increased Proteome Coverage for High-Throughput Studies." Journal of Proteome Research 9(2):997-1006. doi:10.1021/pr900888b
Poster presentation at the 57th conference of the American Society for Mass Spectrometry held at Philadelphia PA