Application of High-Throughput Proteomics
EMSL Project ID
18427
Abstract
The understanding of how cells function at the systems level will greatly benefit from the development of new approaches capable of making global measurements of protein expression (proteome). The aim of this project is to apply new capabilities that are being developed for quantitative and high throughput proteomic measurements, based primarily upon high resolution separations combined with the unique high field Fourier transform ion cyclotron resonance mass spectrometry technology developed at PNNL. The project focuses on biological applications involving studies of the proteomes of several microorganisms including Shewanella oneidensis MR1, Deinococcus radiodurans R1, Rhodobacter sphaeroides and Geobacter sulfurreducens that play significant roles in bioremediation, carbon sequestration or radiation resistance. These efforts are proceeding in collaboration with biologists with expertise in each of these biological systems. The Shewanella research is focusing on the characterization of the organism cultured with different electron acceptors and is closely aligned with the Shewanella Federation. Comparative expression studies with D. radiodurans are being conducted in conjunction with Dr. Michael Daly (USUHS) and PNNL microbiologists to determine the affect of metals on radiation resistance. The Rhodobacter project performed in collaboration with Dr. Timothy Donohue and Dr. Samuel Kaplan focuses on life style dependent protein expression patterns. Finally the Geobacter project, in collaboration with Dr. Derek Lovely is characterization of the cytochrome proteins with extension to global proteomics.
Project Details
Start Date
2006-03-16
End Date
2009-03-22
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Mapping the Subcellular Proteome ofShewanella oneidensisMR-1 using Sarkosyl-Based Fractionation and LC−MS/MS Protein Identification
Brown RN, MF Romine, AA Schepmoes, RD Smith, and MS Lipton. 2010. "Mapping the Subcellular Proteome of Shewanella oneidensis MR-1 using Sarkosyl-based fractionation and LC-MS/MS protein identification." Journal of Proteome Research 9(9):4454-4463. doi:10.1021/pr100215h