The biogeochemical mechanisms controlling reduced radionuclide particle formation properties and stability.
EMSL Project ID
18595
Abstract
To further understand the mechanisms of anaerobic radionuclide reduction, important issues regarding the subcellular localization of reduced, insoluble metal oxides in association with the outer membrane need to be resolved. To address these questions, we will use established anoxic sample preparation techniques to preserve the delicate interaction between biological membranes and the reduced metal oxides. These samples will be sectioned and visualized at high resolution. by TEM. In addition, we have generated polyclonal antibodies specifically recognizing epitopes of proteins associated with metal reduction. Sera generated towards these proteins will be used to determine the subcellular localization of these proteins using immunogold-labeling methods and high resolution transmission electron microscopy. For localization of proteins associated with internal bacterial structures, cells will be fixed, embedded in gelatin, and flash frozen. The ultra thin cryosections will be obtained, mounted on a grid, and incubated using our established immuno-EM hybridization protocols.
Project Details
Project type
Exploratory Research
Start Date
2006-06-09
End Date
2007-07-06
Status
Closed
Released Data Link
Team
Principal Investigator
Related Publications
Citation: Marshall MJ, Beliaev AS, Dohnalkova AC, Kennedy DW, Shi L, et al. (2006) c-Type cytochrome-dependent formation of U(IV) nanoparticles by Shewanella oneidensis.
PLoS Biol 4(8): e268. DOI: 10.1371/journal.pbio.0040268
Marshall MJ, Dohnalkova AC, Kennedy DW, Plymale AE, Thomas SH, Löffler FE, Sanford RA, Zachara JM, Fredrickson JK, Beliaev AS. 2009. Electron donor-dependent radionuclide reduction and nanoparticle formation by Anaeromyxobacter dehalogenans strain 2CP-C. Environ Microbiol doi:10.1111/j.1462-2920.2008.01795.x
Matthew J. Marshall, Andrew E. Plymale, David W. Kennedy, Liang Shi, Zheming Wang, Samantha B. Reed, Alice C. Dohnalkova, Cody J. Simonson, Chongxuan Liu, Daad A. Saffarini, Margaret F. Romine, John M. Zachara, Alexander S. Beliaev, James K. Fredrickson
Hydrogenase- and outer membrane c-type cytochrome-facilitated reduction of technetium(VII) by Shewanella oneidensis MR-1
Environmental Microbiology (OnlineEarly Articles).
doi:10.1111/j.1462-2920.2007.01438.x