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The biogeochemical mechanisms controlling reduced radionuclide particle formation properties and stability.


EMSL Project ID
18595

Abstract

To further understand the mechanisms of anaerobic radionuclide reduction, important issues regarding the subcellular localization of reduced, insoluble metal oxides in association with the outer membrane need to be resolved. To address these questions, we will use established anoxic sample preparation techniques to preserve the delicate interaction between biological membranes and the reduced metal oxides. These samples will be sectioned and visualized at high resolution. by TEM. In addition, we have generated polyclonal antibodies specifically recognizing epitopes of proteins associated with metal reduction. Sera generated towards these proteins will be used to determine the subcellular localization of these proteins using immunogold-labeling methods and high resolution transmission electron microscopy. For localization of proteins associated with internal bacterial structures, cells will be fixed, embedded in gelatin, and flash frozen. The ultra thin cryosections will be obtained, mounted on a grid, and incubated using our established immuno-EM hybridization protocols.

Project Details

Project type
Exploratory Research
Start Date
2006-06-09
End Date
2007-07-06
Status
Closed

Team

Principal Investigator

Matthew Marshall
Institution
Pacific Northwest National Laboratory

Related Publications

Citation: Marshall MJ, Beliaev AS, Dohnalkova AC, Kennedy DW, Shi L, et al. (2006) c-Type cytochrome-dependent formation of U(IV) nanoparticles by Shewanella oneidensis. PLoS Biol 4(8): e268. DOI: 10.1371/journal.pbio.0040268
Marshall MJ, Dohnalkova AC, Kennedy DW, Plymale AE, Thomas SH, Löffler FE, Sanford RA, Zachara JM, Fredrickson JK, Beliaev AS. 2009. Electron donor-dependent radionuclide reduction and nanoparticle formation by Anaeromyxobacter dehalogenans strain 2CP-C. Environ Microbiol doi:10.1111/j.1462-2920.2008.01795.x
Matthew J. Marshall, Andrew E. Plymale, David W. Kennedy, Liang Shi, Zheming Wang, Samantha B. Reed, Alice C. Dohnalkova, Cody J. Simonson, Chongxuan Liu, Daad A. Saffarini, Margaret F. Romine, John M. Zachara, Alexander S. Beliaev, James K. Fredrickson Hydrogenase- and outer membrane c-type cytochrome-facilitated reduction of technetium(VII) by Shewanella oneidensis MR-1 Environmental Microbiology (OnlineEarly Articles). doi:10.1111/j.1462-2920.2007.01438.x