Development of an integrated atomic force / fluorescence microscope at EMSL
EMSL Project ID
19819
Abstract
We propose to develop a new spectroscopic/microscopic capability at EMSL that combines atomic force microscopy (AFM) with fluorescence microscopy allowing for simultaneous force and fluorescence spectroscopic and microscopic measurements on biological samples, such as cells, proteins, and DNA. This new system will consist of an Asylum MFP-3D atomic force microscope, a Coherent Sabre argon ion laser (or a 76 MHz Clark-Spectra-Physics Millennia Nd:YO4 laser pumped Clark-MXR NJA-5 Ti:Sapphire laser, or a Coherent Vanguard 76 MHz Nd:YAG laser pumped dye laser system), a Nikon TE300 (or TE2000U) inverted optical microscope, a Princeton Instrument intensified CCD detector, and an Acton-Research spectrograph. All of these components are currently available and housed in EMSL Environmental Spectroscopy Laboratory (Room 1410). This scheme will offer the ability to acquire simultaneous, directly correlated topographical and optical images of biological samples with high chemical sensitivity and nanometer resolution.
Project Details
Project type
Large-Scale EMSL Research
Start Date
2006-08-01
End Date
2008-08-18
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Lower B. H., R. Yongsunthon, L. Shi, L. Wildling, H. J. Gruber, N. S. Wigginton, C. L. Reardon, G. E. Pinchuk, T. C. Droubay, J-F. Boily, and S. K. Lower. 2009. "Antibody Recognition Force Microscopy Shows that Outer Membrane Cytochromes OmcA and MtrC Are Expressed on the Exterior Surface of Shewanella oneidensis MR-1." Applied and Environmental Microbiology 75:2931-2935.
Ruchirej Yongsunthon, Brian H. Lower, Vance G. Fowler, Emily Alexander, and Steven K. Lower (2008). "A Bacterium's Sense of "Touch"," Materials Research Society Proceedings of Spring 2008 Symposium.