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(emslcsd4)Graphic presentation of conformational motions of dye-labeled enzyme


EMSL Project ID
2179

Abstract

We have studied the conformation change of enzyme molecules by single molecule spectroscopy by monitoring the energy transfer efficiency of dyes labeled on the enzyme. In additional to spectroscopy study, we would like to visualize the conformational motions of the enzyme by computer. The software Insight II in the graphic lab is prefect for this proposes. The enzyme structures are from Protein Data Bank. They will be modified based on the labeling dye and substrate. The graphic presentation will clearly demonstrate how the enzymatic reactivity is related to the conformation.

Project Details

Project type
Capability Research
Start Date
2001-02-12
End Date
2002-10-01
Status
Closed

Team

Principal Investigator

Dehong Hu
Institution
Environmental Molecular Sciences Laboratory

Team Members

H. Peter Lu
Institution
Bowling Green State University

Related Publications

40) Nikolay G. Petrik, Alexander Kavetsky and Greg A. Kimmel, “Electron-Stimulated Production of Molecular Oxygen in Amorphous Solid Water,” J. Phys. Chem. B. 110, 2723 (2006).
42) Nikolay G. Petrik, Alexander Kavetsky and Greg A. Kimmel, “Electron-stimulated production of molecular oxygen in amorphous solid water on Pt(111): Precursor transport through the hydrogen bonding network,” J. Chem. Phys. 125, 124702 (2006).
Kimmel GA, NG Petrik, Z Dohnalek, and BD Kay. "Crystalline Ice Growth on Pt(111) and Pd(111): Nonwetting Growth on a Hydrophobic Water Monolayer." Journal of Chemical Physics 126(11):Art. No. 114702.
Zhou Z, RA Friesner, and L Brus. 2003. "Electronic Structure of 1 to 2 nm Diameter Silicon Core/Shell Nanocrystals: Surface Chemistry, Optical Spectra, Charge Transfer, and Doping." Journal of the American Chemical Society 125(50):15599-15607.