Proteomic Characterization of subprotomes of Shewanella oneidensis
EMSL Project ID
2375
Abstract
Scientists have extensively investigated the biogeochemical processes controlling the reduction of multivalent-metals, including solid phase Fe and Mn oxides, by metal-reducing Shewanella. Yet, relatively little is known regarding the specific metabolic systems involved in metal reduction, let alone the terminal reductases involved in metal reduction. This project will probe the MR-1 networks responsible for its metabolic versatility, focusing initially on metal reduction and attachment to surfaces; the latter believed to be critical function for Fe and Mn oxide reduction. Specifically, we will define the expression of MR-1 genes under tightly controlled culture conditions (e.g., anaerobic metal respiration, adhesion to Fe oxides) by quantifying the proteome using high throughput methods.
Project Details
Project type
Exploratory Research
Start Date
2002-02-28
End Date
2003-10-13
Status
Closed
Released Data Link
Team
Principal Investigator