Platelet proteome for normal and type-2 diabetics
EMSL Project ID
24693
Abstract
Abstract
Our proposal entitled "Platelet proteome for normal and type-2 diabetics" is funded by NIH through an R21 grant. The research is designed to characterize the proteome from human platelets and is important because millions of Americans die or are disabled each year as a consequence of pathologic platelet activation. Activated platelets release bioactive molecules including pro-inflammatory factors that are also thought to play a key role in the mediation of chronic inflammation. Unwanted platelet activation has been implicated in the promotion of cardiovascular disease and type-2 diabetes. Platelets also play a fundamental role in diseases such as myocardial infarction, stroke and deep vein thrombosis. Furthermore, we recently found that inhaled ultra-fine particles, present in polluted air, lead to platelet activation and an inflammatory response in patients with coronary heart disease. Because of the correlation between platelet activation and disease, there is a critical need to better understand platelet function and develop new insights into their proteome—leading to the development of new therapeutic strategies and disease biomarkers. Toward this end our multidisciplinary team will determine the normal platelet proteome and will test the hypothesis that the platelet proteome differs between normal and activated states. In the first aim we will determine the platelet proteome of fresh normal platelets versus type-2 diabetic platelets and in the second we will determine the extent of activation in platelets samples from normal and type-2 diabetic during storage prior to transfusion.
This is a proposal to the Environmental Molecular Sciences Laboratory (EMSL) 2nd Science Theme call for User Proposals. The proposal fits under the "Biological Interactions and Interfaces" science theme, requires "standard access," and is "non-proprietary". This request is for 50 hrs of LTQ time to prepare the PMT database and 60 hrs of Orbitrap time to identify proteins with altered abundance.
Project Details
Project type
Large-Scale EMSL Research
Start Date
2007-06-06
End Date
2009-07-20
Status
Closed
Released Data Link
Team
Principal Investigator
Related Publications
"Platelet Proteome Changes Associated with Diabetes and during Platelet Storage for Transfusion"
David L. Springer, John H. Miller, Sherry L. Spinelli, Ljiljana Pasa-Tolic,
Samuel O. Purvine, Donald S. Daly, Richard C. Zangar, Shuangshuang Jin, Neil Blumberg,
Charles W. Francis, Mark B. Taubman, Ann E. Casey, Steven D. Wittlin, and
Richard P. Phipps
Published on March 6, 2009 on http://pubs.acs.org | doi: 10.1021/pr800885j