Nanoparticles-Based Biosensors for Detection of Environmental Biomarkers
EMSL Project ID
24822
Abstract
Human exposure to toxic chemical agents (i.e. pesticides, nerve agents) and infectious agents, such as pathogens, may have some immune response, which is composed of both cellular and acellular components. Protein biomarkers are biomolecular signatures that display a unique pattern of molecular change in an organism, such as a set of expressed proteins, and that identify an exposure or response to a specific environmental stressor. Biological pathways that characterize organism response to environmental or biological agent exposures will be identified under the Environmental Biomarkers Initiative (EBI) at PNNL and 3 NIH funded projects. Moving this information to application will require methods of detection (i.e., biosensors) for biomarkers or biosignature patterns which are under discovery in several ongoing EBI projects and NIH funded projects (PI, Yuehe Lin). The EBI leadership has chosen to create this parallel path for sensor development in an effort to improve the Laboratorys ability to characterize and compare EBI-relevant detection methods. The major leap forward that has to occur is to also demonstrate that the new bioassays or devices will be more portable, sensitive, selective and real time than methods requiring large fluorescent microarray readers or flow cytometric equipment.
Project Details
Project type
Large-Scale EMSL Research
Start Date
2007-06-08
End Date
2010-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Barry RC, Y Lin, J Wang, G Liu, and C Timchalk. 2009. "Nanotechnology-Based Electrochemical Sensors for Biomonitoring Chemical Exposures ." Journal of Exposure Science and Environmental Epidemiology 19:1-18. doi:10.1038/jes.2008.71
Du D, J Wang, L Wang, D Lu, JN Smith, C Timchalk, and Y Lin. 2011. "Magnetic Electrochemical Sensing Platform for Biomonitoring of Exposure to Organophosphorus Pesticides and Nerve Agents Based on Simultaneous Measurement of Total Enzyme Amount and Enzyme Activity ." Analytical Chemistry 83(10):3770-3777. doi:10.1021/ac200217d
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Lee AC, G Liu, CK Heng, SN Tan, TM Lim, and Y Lin. 2008. "Sensitive electrochemical detection of horseradish peroxidase at disposable screen-printed carbon electrode." Electroanalysis 20(18):2040-2046. doi:DOI: 10.1002/elan.200804287
Lee AC, Z Dai, B Chen, H Wu, J Wang, A Zhang, L Zhang, TM Lim, and Y Lin. 2008. "Electrochemical Branched-DNA Assay for Polymerase Chain Reaction-Free Detection and Quantification of Oncogenes in Messenger RNA." Analytical Chemistry 80(24):9402-9410. doi:10.1021/ac801263r
Lin Y, and HS Nalwa. 2009. Handbook of Electrochemical Nanotechnology, vol. 1-2, American Scientific Publishers, Stevenson Ranch, CA.
Lin Y, D Choi, J Wang, and JR Bontha. 2009. "Nanomaterials-Enhanced Electrically Switched Ion Exchange Process for Water Treatment." Chapter 14 in Nanotechnology Applications for Clean Water, ed. M. Savage, et al, pp. 179-189. William Andrew, Norwich, NY.
Lin YY, G Liu, CM Wai, and Y Lin. 2008. "Bioelectrochemical Immunoassay of Polychlorinated Biphenyl." Analytica Chimica Acta 612:23-28. doi:10.1016/j.aca.2008.01.080
Lin YY, J Wang, G Liu, H Wu, CM Wai, and Y Lin. 2008. "A nanoparticle label/immunochromatographic electrochemical biosensor for rapid and sensitive detection of prostate-specific antigen ." Biosensors and Bioelectronics 23(11):1659-1665.
Liu G, J Wang, RC Barry, CE Petersen, C Timchalk, PL Gassman, and Y Lin. 2008. "Nanoparticle-Based Electrochemical Immunosensor for the Detection of Phosphorylated Acetylcholinesterase Adduct: An Exposure Biomarker of Organophosphate Pesticides and Nerve Agents." Chemistry - a European Journal 14(32):9951-9959. doi:10.1002/chem.200800412
Miao Y, H Wang, Y Shao, Z Tang, J Wang, and Y Lin. 2009. "Layer-By-Layer Assembled Hybrid Film of Carbon Nanotubes/Iron Oxide Nanocrystals for Reagentless Electrochemical Detection of H2O2." Sensors and Actuators. B, Chemical 138(1):182-188.
Shao Y, J Liu, Y Wang, and Y Lin. 2008. "Novel Catalyst Support Materials for PEM Fuel Cells: Current Status and Future Prospects." Journal of Materials Chemistry 19(1):46-59. doi:10.1039/b808370c
Tang Z, H Wu, D Du, J Wang, H Wang, W Qian, DJ Bigelow, JG Pounds, RD Smith, and Y Lin. 2010. "Sensitive Immunoassays of Nitrated Fibrinogen in Human Biofluids." Talanta 81(4-5):1662-1669.
Tang Z, H Wu, JR Cort, GW Buchko, Y Zhang, Y Shao, IA Aksay, J Liu, and Y Lin. 2010. "Constraint of DNA on Functionalized Graphene Improves Its Biostability and Specificity." Small 6(11):1205-1209. doi:10.1002/smll.201000024
Wang H, J Wang, C Timchalk, and Y Lin. 2008. "Magnetic Electrochemical Immunoassays with Quantum Dot Labels for Detection of Phosphorylated Acetylcholinesterase in Plasma." Analytical Chemistry 80(22):8477-8484. doi:10.1021/ac801211s
Wang H, J Wang, D Choi, Z Tang, H Wu, and Y Lin. 2009. "EQCM Immunoassay for Phosphorylated Acetylcholinesterase as a Biomarker for Organophosphate Exposures Based on Selective Zirconia Adsorption and Enzyme-Catalytic Precipitation ." Biosensors and Bioelectronics 24(8):2377-2383.
Wang J, and Y Lin. 2008. "Functionalized carbon nanotubes and nanofibers for biosensing applications." Trends in Analytical Chemistry. TrAC 27(7):619-626. doi:10.1016/j.trac.2008.05.009
Wang J, and Y Lin. 2009. "Nanomaterial-Based Biosensors for Detection of Pesticides and Explosives." Chapter 26 in Nanotechnology Applications for Clean Water, ed. N. Savage, et al, pp. 377-390. William Andrew, Nowich, NY.
Wang J, C Timchalk, and Y Lin. 2008. "Carbon Nanotube-Based Electrochemical Sensor for Assay of Salivary Cholinesterase Enzyme Activity: An Exposure Biomarker of Organophosphate Pesticides and Nerve Agents." Environmental Science & Technology 42(7):2688-2693. doi:10.1021/es702335y
Wang J, G Liu, H Wu, and Y Lin. 2009. "Biosensors Based on Functionalized Carbon Nanotubes, Nanoparticles, and Nanowires." Chapter 4 in Handbook of Electrochemical Nanotechnology, vol. II, ed. Yuehe Lin and H. S. Nalwa, pp. 95-111. American Scientific Publishers, Stevenson Ranch, CA.
Wang S, X Zhang, X Mao, Q Zeng, H Xu, Y Lin, W Chen, and G Liu. 2008. "Electrochemical Immunoassay of Carcinoembryonic Antigen Based on A Lead Sulfide Nanoparticle Label." Nanotechnology 19(43):Art. No. 435501. doi:10.1088/0957-4484/19/43/435501
Wang Y, Y Shao, DW Matson, J Li, and Y Lin. 2010. "Nitrogen-Doped Graphene and its Application in Electrochemical Biosensing." ACS Nano 4(4):1790-1798.
Wu H, J Wang, Z Wang, DR Fisher, and Y Lin. 2008. "Apoferritin-Templated Yttrium Phosphate Nanoparticle Conjugates for Radioimmunotherapy of Cancers." Journal of Nanoscience and Nanotechnology 8(5):2316-2322. doi:10.1166/jnn.2008.177
Wu H, MH Engelhard, J Wang, DR Fisher, and Y Lin. 2008. "Synthesis of Lutetium Phosphate/Apoferritin Core-Shell Nanoparticles for Potential Applications in Radioimmunoimaging and Radioimmunotherapy of Cancers." Journal of Materials Chemistry 18(15):1779-1783.
Wu H, Q Huo, SM Varnum, G Liu, J Wang, Z Nie, J Liu, and Y Lin. 2008. "Dye-Doped Silica Nanoparticle Labels/Protein Microarray for Detection of Protein Biomarkers." The Analyst 133:1550 - 1555. doi:10.1039/b719810h
Zhang Y, Z Tang, J Wang, H Wu, A Ma Ham, and Y Lin. 2010. "Hairpin DNA Switch for Ultrasensitive Spectrophotometric Detection of DNA Hybridization Based on Gold Nanoparticles and Enzyme Signal Amplification." Analytical Chemistry 82(15):6440–6446. doi:10.1021/ac1006238