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Development of Single Molecule Force Microscopy Capability to Examine the Moleuclar Mechanism of Lignocellulosic Material Degradation


EMSL Project ID
25425

Abstract

The objective of this proposal is to develop a novel capability based on scanning probe microscopy to examine the enzyme-catalyzed hydrolysis of insoluble lignocellulosic material at the molecular level. Single-molecule-recognition atomic force microscopy (AFM) will be developed and used to examine the organization and interactions of individual protein molecules with respect to plant cell architecture and relative to the lignocellulosic material during enzymatic hydrolysis. This new capability will enable insight into the enzymatic degradation of lignocellulose at a fundamental level of detail to ultimately allow the development of strategies to improve the efficiency of lignocellulosic degradation via the synergetic actions of cellulases, hemicellulases, and ligninases.

Project Details

Project type
Large-Scale EMSL Research
Start Date
2007-08-01
End Date
2008-09-26
Status
Closed

Team

Principal Investigator

Brian Lower
Institution
The Ohio State University

Team Members

Ziyu Dai
Institution
Pacific Northwest National Laboratory

Galya Orr
Institution
Environmental Molecular Sciences Laboratory

Zheming Wang
Institution
Pacific Northwest National Laboratory

Kevin Rosso
Institution
Pacific Northwest National Laboratory

Related Publications

Lower B. H., R. Yongsunthon, L. Shi, L. Wildling, H. J. Gruber, N. S. Wigginton, C. L. Reardon, G. E. Pinchuk, T. C. Droubay, J-F. Boily, and S. K. Lower. 2009. "Antibody Recognition Force Microscopy Shows that Outer Membrane Cytochromes OmcA and MtrC Are Expressed on the Exterior Surface of Shewanella oneidensis MR-1." Applied and Environmental Microbiology 75:2931-2935.