Developing Calculation Methods to Precisely Measure Proteome Proteins and Their Post-Translational Modifications
EMSL Project ID
26194
Abstract
In this proposed work we intend to: 1) Make an UStag caluclation algorithm into a high-throughput format that would be user friendly fo rthe general proteome community; 2) Create a human post translational modification (PTM) library containing accurate mass and elution time information; 3) Examine human samples for mutations and variations from the human genome; 4) Develop distributed and/or parallel algorithms needed to overcome computational challenges needed for high throughput automated application of UStag method.
Project Details
Start Date
2007-04-26
End Date
2010-05-02
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Proteome-Wide Identification of Proteins and Their Modifications with Decreased Ambiguities and Improved False Discovery Rates Using Unique Sequence Tags
Shen, Y., Tolic, N., Hixson, K.K., Purvine, S.O., Pasa-Tolic, L., Qian, W.-J., Adkins, J.N., Moore, R.J., and Smith, R.D.
Anal. Chem., 2008, 10.1021/ac702328x
Shen Y, KK Hixson, N Tolic, DG Camp, II, SO Purvine, RJ Moore, and RD Smith. 2008. "Mass spectrometry analysis of proteome-wide proteolytic post-translational degradation of proteins." Analytical Chemistry 80(15):5819-5828. doi:10.1021/ac800077w
Shen Y, N Tolic, KK Hixson, SO Purvine, GA Anderson, and RD Smith. 2008. "De novo sequencing of unique sequence tags for discovery of post-translational modifications of proteins ." Analytical Chemistry 80(20):7742-7754. doi:10.1021/ac801123p