Post mortem energy metabolism and water characteristics in rabbit m. longissimus studied by dynamic slow-speed MAS NMR spectroscopy and relaxometry
EMSL Project ID
2646
Abstract
Using a slow-speed (about 40-100 Hz) magic angle spinning technique (PASS), the aim of the present study present study is to follow the post mortem course in rabbit muscle biopsies from proton NMR spectra measured continuously during the post mortem period (from for example 20 min post mortem and until 12-18 h post mortem). In particular, the goal is to follow the formation of lactate, and eventually, to follow the simultaneous degradation of phosphocreatine and ATP post mortem by the application of combined 31P NMR spectroscopy. In order to create a variation in the formation of lactate in the investigated muscles, before killing half the rabbits are administered adrenaline, which causes depletion of muscle glycogen stores and thereby prevents formation of lactate post mortem. The study is performed by continuously measurement of low-speed MAS proton NMR spectra on excised muscle tissue during the early conversion of muscle to meat, i.e. early as possible after death and until about 12 h post mortem. Spectra are recorded using the PASS sequence, and in addition, the PASS sequence is planned extended with the CPMG sequence to measure the transverse relaxation times. The obtained data is planned related to meat quality, and as a measure of meat quality, water-holding capacity of the meat is determined by centrifugation of samples taken 24 h post mortem.
Project Details
Project type
Capability Research
Start Date
2003-03-15
End Date
2003-09-16
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Bertram H, J Hu, DN Rommereim, RA Wind, and HJ Andersen. 2004. "Dynamic High-Resolution H-1 and P-31 NMR Spectroscopy and H-1 T-2 Measurements in Postmortem Rabbit Muscles Using Slow Magic Angle Spinning." Journal of Agricultural and Food Chemistry 52(9):2681-2688.