Proteomic Studies of Inflammation and the Host Response to Injury
EMSL Project ID
29404
Abstract
Inflammation and The Host Response to Injury, a large scale collaborative research program, is currently funded as a "glue" grant by the National Institute of General Medical Sciences (NIGMS). Proteomics is one of the core components of this research program. The focus of this user proposal isto utilize high resolution liquid chromatography-mass spectrometry for quantitative analyses of the changes in blood leukocyte and plasma protein concentration that accompany traumatic and burn injury. Specifically, Stable isotope labeling and the accurate mass and time tag approach using LC-FTICR will be combined as a high throughput proteomic strategy to survey the leukocyte proteomes and plasma proteome from a large number of clinical samples and to discover novel biomarkers to inflammation and injury. Clinical samples from trauma patients at different time-point of treatment will be evaluated and the global
proteomic data with be integrated with functional genomic data. Due to the challenge of dynamic range and sample complexity, methods in sample preparation, separation, and
instrumentation will be evaluated or refined to improve the overall dynamic range of the detection in order to discover novel low abundance proteins. Bioinformatics tools such as feature comparison or identification across multiple clinical samples will also be utilized or refined to obtain useful data from these analyses.
The long term goal of this study is to obtain an unprecedented analysis of the proteomic responses to serious traumatic or burn injury. Furthermore, these studies are aimed at identifying
changes in the levels of these proteins that may predict which clinical trajectory these patients will follow, and provide fundamental biologic information concerning the immuno-inflammatory response to traumatic and burn injury.
Project Details
Start Date
2008-03-06
End Date
2011-03-13
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Kotz KT, W Xiao, C Miller-Graziano, W Qian, A Russom, EA Warner, LL Moldawer, A De, PE Bankey, BO Petritis, DG Camp, II, AE Rosenbach, J Goverman, SP Fagan, BH Brownstein, D Irimia, W Xu, J Wilhelmy, M Mindrinos, RD Smith, RW Davis, RG Tompkins, and M Toner. 2010. "Clinical Microfluidics for Neutrophil Genomics and Proteomics." Nature Medicine 16(9):1042-1048. doi:10.1038/nm.2205
Qian W, BO Petritis, A Kaushal, CC Finnerty, MG Jescheke, ME Monroe, RJ Moore, AA Schepmoes, W Xiao, LL Moldawer, RW Davis, RG Tompkins, DN Herndon, DG Camp, II, and RD Smith. 2010. "Plasma Proteome Response to Severe Burn Injury Revealed by 18O-Labeled “Universal” Reference-based Quantitative Proteomics." Journal of Proteome Research 9(9):4779-4789.
Qian W, DT Kaleta, BA Ogata, H Jiang, T Liu, X Zhang, HM Mottaz, SM Varnum, DG Camp, II, Huang, X Fang, W Zhang, and RD Smith. 2008. "Enhanced Detection of Low Abundant Human Plasma Proteins using a Tandem IgY12-SuperMix Immunoaffinity Separation Strategy." Molecular & Cellular Proteomics. MCP 7(10):1963-1973. doi:10.1074/mcp.M800008-MCP200
Qian, W.J.; Liu, T.; Petyuk, V.A.; Gritsenko, M.A.; Petritis, B.O.; Polpitiya, A.D.; Kaushal, A.; Xiao, W.; Finnerty, C.C.; Jeschke, M.G.; Jaitly, N.; Monroe, M.E.; Moore, R.J.; Moldawer, L.L.; Davis, R.W.; Tompkins, R.G.; Herndon, D.N.; Camp, D.G. and Smith, R.D. Large-Scale Multiplexed Quantitative Discovery Proteomics Enabled by the Use of an (18)O-Labeled "Universal" Reference Sample. J. Proteome Res., 2009, 8, 290-299.