Proteomic Characterization of In Vivo and In Vitro Model Systems of Hepatitis C Virus Infection:
EMSL Project ID
29794
Abstract
This EMSL user proposal represents a continuation of our proteomic efforts aimed at understanding HCV replication and pathogenesis. The work proposed here represents a collaborative effort with Dr. Richard Smith at Pacific Northwest National Laboratory (PNNL) that is supported by the successful competitive renewal of a Program Project Grant from the National Institute on Drug Abuse entitled "Functional Genomics and HCV-Associated Liver Disease" that began funding June 1, 2007 (grant 1P30DA01562501 to M.G.K.). The overall goals of EMSL User Proposal is to: 1) further augment the informatics and proteomics resources necessary for ultra-sensitive, high-throughput quantitative protein profiling studies using human liver biopsy specimens, and 2) leverage continued advances in instrumentation and methodologies to characterize new model systems (e.g. the SCID-beige/Alb-uPA mouse model of HCV infection and the in vitro HCV 2a infection system) and additional clinical samples (e.g. serial plasma samples collected in parallel with serial liver biopsy samples from patients with recurrent HCV infection after liver transplantation).
Project Details
Start Date
2008-05-01
End Date
2011-05-08
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Diamond DL, AJ Syder, JM Jacobs, CM Sorensen, KA Walters, S Proll, JE McDermott, MA Gritsenko, Q Zhang, R Zhao, TO Metz, DG Camp, II, KM Waters, RD Smith, CM Rice, and MG Katze. 2010. "Temporal Proteome and Lipidome Profiles Reveal HCV-Associated Reprogramming of Hepatocellular Metabolism and Bioenergetics ." PLoS Pathogens 6(1):Art. No. e1000719. doi:10.1371/journal.ppat.1000719
McDermott JE, DL Diamond, CD Corley, A Rasmussen, MG Katze, and KM Waters. 2012. "Topological Analysis of Protein Co-Abundance Networks Identifies Novel Host Targets Important for HCV Infection and Pathogenesis ." BMC Systems Biology 6(1):28. doi:10.1186/1752-0509-6-28