Photoswitchable Dyes and Nanoparticles Enable High Resolution and Accurate Fluorescent Probes for Cell Imaging
EMSL Project ID
30409
Abstract
We have developed photoswitchable dyes and nanoparticles for high-resolution and high-accuracy cell imaging. Upon photoactivation, the fluorescent molecules switch either emission colors or between bright and dark states. We will apply the photoswitchable molecules and nanoparticles to advance microscopy techniques. Both on-and-off switching and dual color imaging will enhance image contrast and reveal nano-scale interaction in cells. STORM will dramatically improve the spatial resolution of cell imaging to the nanometer scale, far beyond the diffraction limit. Using these new microscopy techniques, we will track HMGA1a receptors location and density down to nano-scales on cell surfaces and into cytoplasm. We will also study the endocytosis process of nanoparticles into cells and the fate of the nanoparticles.
Project Details
Project type
Large-Scale EMSL Research
Start Date
2008-08-19
End Date
2011-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Photoswitchable Nanoprobes Offer Unlimited Brightness in Frequency-Domain Imaging
Li AD, C Zhan, D Hu, W Wan, and J Yao. 2011. "Photoswitchable Nanoprobes Offer Unlimited Brightness in Frequency-Domain Imaging." Journal of the American Chemical Society 133(20):7628-7631. doi:10.1021/ja1108479
Nanometer resolution imaging by single molecule switching
Hu D, and G Orr. 2010. "Nanometer Resolution Imaging by Single Molecule Switching." Nano Reviews 1:5122. doi:10.3402/nano.v1i0.5122
Photoswitchable Nanoparticles Enable High-Resolution Cell Imaging: PULSAR Microscopy
Hu D, Z Tian, W Wu, W Wan, and AD Li. 2008. "Photoswitchable Nanoparticles Enable High-Resolution Cell Imaging: PULSAR Microscopy." Journal of the American Chemical Society 130(46):15279-15281. doi:10.1021/ja805948u