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Alpha Project


EMSL Project ID
3162

Abstract

The Alpha Project employs a multidisciplinary approach that focuses on the prediction of quantitative events in a biological system through the investigation of functional genomics in combination with computational research specifically applied to the G-protein receptor-coupled signal transduction pathway (STP) governing response to the alpha factor mating pheromone of haploid yeast cells. Experimental methods for the quantitation of the alpha factor-induced cellular response, including the formation of key intermediates in the STP, will be developed and monitored by the extremely sensitive and high-resolution method of reversed phase capillary liquid chromatography coupled with electrospray ionization Fourier transform ion cyclotron tandem mass spectrometry (LC-FTICR MS/MS) at Pacific Northwest National Laboratory, Richland, Washington. The detection, identification, and quantitation of specific protein phosphorylation events in the STP are key to unraveling the effects of defined perturbations to the yeast cells. A limited accurate mass and time (AMT) tag database will be developed for the posttranslationally modified and unmodified proteins in the STP.

Project Details

Project type
Exploratory Research
Start Date
2002-12-05
End Date
2005-12-11
Status
Closed

Team

Principal Investigator

David Camp
Institution
Pacific Northwest National Laboratory