Deinococcus radiodurans
EMSL Project ID
3203
Abstract
The most significant defining characteristic of D. radiodurans is its ability to resist the lethal effects of DNA damaging agents such as ionizing radiation, UV radiation, hydrogen peroxide and desiccation. The capacity for survival after severe DNA damage at such high levels of ionizing radiation is apparently the result of unusually efficient repair mechanisms. The ability to understand these biological systems and their constituents would be greatly facilitated by the ability to make quantitative, sensitive, and comprehensive measurements of how their proteome changes in response to environmental perturbations. To this end we will use the new instrumentation and a high throughput methodology developed in the EMSL to characterize an organism’s dynamic proteome based upon the combination of global enzymatic digestion, high-resolution liquid chromatographic separations and analysis by Fourier transform ion cyclotron resonance mass spectrometry.
Project Details
Project type
Exploratory Research
Start Date
2002-12-05
End Date
2004-09-27
Status
Closed
Released Data Link
Team
Principal Investigator
Related Publications
Himmelberger, D. W.; Alden, L. R.; Bluhm, M. E.; Sneddon, L. G. Inorg. Chem. 2009, 48, 9883-9889.
Himmelberger, D. W.; Yoon, C. W.; Bluhm, M. E.; Carroll, P. J.; Sneddon, L. G. J. Am. Chem. Soc. 2009, 131, 14101-14110.