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Immobilized Enzymes for Bioremediation and Biosensing - Armored Enzyme Nanoparticles for Remediation of Subsurface Contaminants (JB's EMSP Project - 43946) and Nanoscale Enzyme Reactors (JB's DARPA Project - 49090)


EMSL Project ID
3241

Abstract

Immobilized enzyme systems, stabilizing the enzyme activity, will be tested for the purposes of bioremediation and biosensing. Stabilization of enzyme activity is a critical advance that is required in order to use enzymes effectively for the remediation and/or biosensing of organic compounds in various environments. Various techniques of enzyme immobilizaiton will be investigated and compared, beginning with a protease (alpha-chymotrypsin) and tyrosinase, both of which are commercially available. We may also use a haloalkane dehalogenase for the degradation of trichloroethylene (TCE) and other chlorinated hydrocarbons of interest to DOE.

Project Details

Project type
Capability Research
Start Date
2003-01-16
End Date
2006-01-22
Status
Closed

Team

Principal Investigator

Jungbae Kim
Institution
Pacific Northwest National Laboratory

Team Members

Sun-ae Jun
Institution
Dongguk University

Sang-mok Lee
Institution
Inha University

Xueyan Zhao
Institution
University of Minnesota

Jongkyu Youn
Institution
Korea Advanced Institute of Science and Technology (KAIST)

Beom Kim
Institution
Chungbuk National University

Hye-kyung Ahn
Institution
Chungbuk National University

Byoung Chan Kim
Institution
Gwangju Institute of Science and Technology (GIST)

Sujith Nair
Institution
Pennsylvania State University

Hyon Bin Na
Institution
Seoul National University

Thurston Herricks
Institution
University of Washington

Jinwoo Lee
Institution
Seoul National University

Seung-wook Chung
Institution
Seoul National University

Moon II Kim
Institution
Korea Advanced Institute of Science and Technology (KAIST)

Hongfei Jia
Institution
The University of Akron

Jay Grate
Institution
Pacific Northwest National Laboratory

Kyoung Seon Min
Institution
Seoul National University

Chang Won Lee
Institution
Seoul National University

Related Publications

Biocatalytic Single-Enzyme Nanoparticles
Herricks TE, SH Kim, J Kim, D Li, J Kwak, JW Grate, and Y Xia. 2005. "Direct Fabrication of Enzyme-Carrying Polymer Nanofibers by Electrospinning." Journal of Materials Chemistry 15(31):3241-3245.
Kim B, S Nair, J Kim, J Kwak, JW Grate, SH Kim, and M Gu. 2005. "Preparation of biocatalytic nanofibers with high activity and stability via enzyme aggregate coating on polymer nanofibers." Nanotechnology 16(7):S382-S388.
Kim J, and JW Grate. 2003. "Single-Enzyme Nanoparticles Armored by a Nanometer-Scale Organic/Inorganic Network." Nano Letters 3(9):1219-1222.
Kim J, J Lee, H Na, B Kim, J Youn, J Kwak, K Moon, E Lee, J Kim, J Park, A Dohnalkova, H Park, M Gu, H Chang, JW Grate, and T Hyeon. 2005. "A Magnetically Separable, Highly Stable Enzyme System Based on Nanocomposites of Enzymes and Magnetic Nanoparticles Shipped in Hierarchically Ordered, Mesocellular, Mesoporous Silica." Small 1(12):1203-1207.
Kim J, JW Grate, and P Wang. 2006. "Nanostructures for enzyme stabilization." Chemical Engineering Science 61(3):1017-1026.
Lee D, J Lee, J Kim, H Na, B Kim, C Shin, J Kwak, A Dohnalkova, JW Grate, T Hyeon, and HSung Kim. 2005. "Simple Fabrication of a Highly Sensitive and Fast Glucose Biosensor using Enzyme Immobilized in Mesocellular Carbon Foam." Advanced Materials 17(23):2828-2833.
Lee J, J Kim, H Jia, M Kim, J Kwak, S Jin, A Dohnalkova, H Park, H Chang, P Wang, JW Grate, and T Hyeon. 2005. "Simple Synthesis of Hierarchically Ordered Mesocellular Mesoporous Silica Materials Hosting Crosslinked Enzyme Aggregates." Small 1(7):744-753.