Immobilized Enzymes for Bioremediation and Biosensing - Armored Enzyme Nanoparticles for Remediation of Subsurface Contaminants (JB's EMSP Project - 43946) and Nanoscale Enzyme Reactors (JB's DARPA Project - 49090)
EMSL Project ID
3241
Abstract
Immobilized enzyme systems, stabilizing the enzyme activity, will be tested for the purposes of bioremediation and biosensing. Stabilization of enzyme activity is a critical advance that is required in order to use enzymes effectively for the remediation and/or biosensing of organic compounds in various environments. Various techniques of enzyme immobilizaiton will be investigated and compared, beginning with a protease (alpha-chymotrypsin) and tyrosinase, both of which are commercially available. We may also use a haloalkane dehalogenase for the degradation of trichloroethylene (TCE) and other chlorinated hydrocarbons of interest to DOE.
Project Details
Project type
Capability Research
Start Date
2003-01-16
End Date
2006-01-22
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Biocatalytic Single-Enzyme Nanoparticles
Herricks TE, SH Kim, J Kim, D Li, J Kwak, JW Grate, and Y Xia. 2005. "Direct Fabrication of Enzyme-Carrying Polymer Nanofibers by Electrospinning." Journal of Materials Chemistry 15(31):3241-3245.
Kim B, S Nair, J Kim, J Kwak, JW Grate, SH Kim, and M Gu. 2005. "Preparation of biocatalytic nanofibers with high activity and stability via enzyme aggregate coating on polymer nanofibers." Nanotechnology 16(7):S382-S388.
Kim J, and JW Grate. 2003. "Single-Enzyme Nanoparticles Armored by a Nanometer-Scale Organic/Inorganic Network." Nano Letters 3(9):1219-1222.
Kim J, J Lee, H Na, B Kim, J Youn, J Kwak, K Moon, E Lee, J Kim, J Park, A Dohnalkova, H Park, M Gu, H Chang, JW Grate, and T Hyeon. 2005. "A Magnetically Separable, Highly Stable Enzyme System Based on Nanocomposites of Enzymes and Magnetic Nanoparticles Shipped in Hierarchically Ordered, Mesocellular, Mesoporous Silica." Small 1(12):1203-1207.
Kim J, JW Grate, and P Wang. 2006. "Nanostructures for enzyme stabilization." Chemical Engineering Science 61(3):1017-1026.
Lee D, J Lee, J Kim, H Na, B Kim, C Shin, J Kwak, A Dohnalkova, JW Grate, T Hyeon, and HSung Kim. 2005. "Simple Fabrication of a Highly Sensitive and Fast Glucose Biosensor using Enzyme Immobilized in Mesocellular Carbon Foam." Advanced Materials 17(23):2828-2833.
Lee J, J Kim, H Jia, M Kim, J Kwak, S Jin, A Dohnalkova, H Park, H Chang, P Wang, JW Grate, and T Hyeon. 2005. "Simple Synthesis of Hierarchically Ordered Mesocellular Mesoporous Silica Materials Hosting Crosslinked Enzyme Aggregates." Small 1(7):744-753.