Development of ETD-enhanced FT-ICR MS
EMSL Project ID
32691
Abstract
The objective of this proposed one-year EMSL-Bruker Daltonics partnership is to incorporate electron transfer dissociation (ETD)-- relatively new dissociation technique--with EMSL's 12-Tesla Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer. The combination of ETD and FT-ICR will enhance the analysis of proteins and protein isoforms in complex biological systems by enabling improved characterization of post-translational modifications (PTMs) to proteins, such as phosphorylation, which modulate the activity and function of proteins in biological systems of interest. The proposed new instrumentation platform has the potential to transform biological and environmental sciences, and the proposed collaboration will make EMSL the site of development and deployment of this transformational tool. ETD will be implemented based upon pre-production hardware and software developed by Bruker Daltonics and made available to PNNL as a collaborative effort to further develop and demonstrate the potential of ETD combined with FT-ICR. The characterization of PTMs of intact proteins from known standards and model biological systems will demonstrate the potential of the ETD FT-ICR platform for EMSL users. Several current EMSL user proposals and recent EMSL user applications would benefit from the availability of the ETD FT-ICR platform, and developing this system will strengthen U.S. scientific foundations for innovation. Through this development in collaboration with Bruker Daltonics, EMSL would gain a unique opportunity to leverage existing technologies in biological separations and proteomics with ETD and create a leading-edge technology in mass spectrometry.
Project Details
Project type
Scientific Partner
Start Date
2009-12-22
End Date
2014-10-11
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Tolmachev AV, EW Robinson, S Wu, RD Smith, and L Pasa-Tolic. 2011. "Trapping radial electric field optimization in compensated FTICR Cells." Journal of the American Society for Mass Spectrometry 22(8):1334-1342. doi:10.1007/s13361-011-0167-z
Tolmachev AV, EW Robinson, S Wu, RD Smith, JH Futrell, and L Pasa-Tolic. 2012. "Angular Averaged Profiling of the Radial Electric Field in Compensated FTICR Cells." Journal of the American Society for Mass Spectrometry. doi:10.1007/s13361-012-0395-x