Development of Live and LC-NMR Microbial Metabolomics Methods for Systems Biology Studies: A Test Case Relevant to BioFuels Production
EMSL Project ID
33391
Abstract
We are requesting access time for two EMSL resources, the Varian 600 MHz metabolomics NMR system and the Bruker Avance 500 MHz MRI system, to perform work for a newly funded EMSL Capability Development Call proposal, "Development of Live and LC-NMR Microbial Metabolomics Methods for Systems Biology Studies: A Test Case Relevant to BioFuels Production". We will partner with the new Bioproducts, Sciences and Engineering Laboratory (BSEL) to develop a new EMSL microbial metabolomics capability to complement existing systems biology approaches in the context of microbial biofuel production systems. Using a promising microbial biofuels production organism, Caldicellulosiruptor saccharolyticus, as a test case, we will integrate EMSL's unique NMR bioreactor technology, providing real-time spectroscopy of living cultures, with EMSL's new 600 MHz metabolomics platform, which provides high resolution, high sensitivity cold probe NMR and LC-NMR methods to identify key metabolites and quantify their metabolic fluxes. The unique combination of bioreactor near-instantaneous in-vivo information, without the need for sample manipulation that can degrade metabolite profiles, with high sensitivity cold probe in-vitro identification of low concentration metabolites and resolution of overlapped metabolites by LC and 2D NMR, is a key feature of our proposed integrated capability. The resulting, enduring EMSL technology will be applicable to and available for a wide range of energy, environmental and health-related studies.
Project Details
Start Date
2009-04-15
End Date
2012-04-15
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Isern NG, J Xue, JV Rao, JR Cort, and BK Ahring. 2013. "Novel monosaccharide fermentation products in Caldicellulosiruptor saccharolyticus identified using NMR spectroscopy." Biotechnology for Biofuels 6(4):Article No. 47. doi:10.1186/1754-6834-6-47
Oliveira F.C., K. Srinivas, G.L. Helms, N.G. Isern, J.R. Cort, A.R. Goncalves, and B.K. Ahring. 2018. "Characterization of coffee (Coffea arabica) husk lignin and degradation products obtained after oxygen and alkali addition." Bioresource Technology 257. PNNL-SA-131064. doi:10.1016/j.biortech.2018.01.041
Xue J, NG Isern, RJ Ewing, AV Liyu, JA Sears, Jr, H Knapp, J Iversen, DR Sisk, BK Ahring, and PD Majors. 2014. "New generation NMR bioreactor coupled with high-resolution NMR spectroscopy leads to novel discoveries in Moorella thermoaceticum metabolic profiles." Applied Microbiology and Biotechnology. doi:10.1007/s00253-014-5847-8