Discovery novel therapeutic protein targets by advanced MS proteomics
EMSL Project ID
33598
Abstract
The objective of this research is to discover novel therapeutic protein targets for human metabolic diseases by applying advanced PNNL proteomics technologies to a variety of tissue, cell, or biofluid samples from animal models or human subjects. Differentially-expressed peptides/proteins in the proteome of RYGB and sham-operated GK or zucker fatty rats will be identified by focusing on the secretome of gut and peptide hormones from blood.
High resolution LC-MS based quantitative proteomics will be applied for this study. The study will be a collaboration with NGM Biopharmaceuticals, who will provide the purified secretory granules from small intestine and enriched peptides from blood for LC-MS analyses.
Project Details
Start Date
2009-08-18
End Date
2012-08-26
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members