Surface-Induced Dissociation of Large Molecules in Mass Spectrometry
EMSL Project ID
39717
Abstract
The general objective of our research is to achieve a fundamental understanding of the reaction kinetics and dynamics of activating and dissociating complex molecular ions, develop molecular level understanding of interactions of complex ions and molecules with surfaces, and develop new approaches for selective modification of substrates using beams of mass-selected hyperthermal ions. Our research addresses analytical challenges relevant to a broad range of applications within the Department of Energy (DOE) mission areas. Understanding ion activation and dissociation provides the scientific foundation for tandem mass spectrometry, one of the most important analytical methods developed in the last century. We investigate collisional activation and dissociation of a variety of complex molecules including protonated and deprotonated peptides, peptide radical cations and anions, non-covalent complexes, and metalloorganic complexes. Characterization of these molecules using tandem mass spectrometry is central to several fields including proteomics and catalysis, and is essential for understanding self assembly and molecular recognition processes. Our findings obtained for these model systems are relevant to a broader class of complex molecules in biofuels, petroleum samples, and constituents of secondary organic aerosols that are of specific interest to DOE. We investigate the dynamics of surface-induced dissociation (SID) of large molecules and the influence of physical and chemical properties of SID targets on the energy transfer into complex ions using time- and collision energy-resolved measurements in FT-ICR MS. Theoretical modeling of experimental data provides information on the energetics and dynamics of complex ion dissociation processes. EMSL provides unique resources necessary for the proposed research.
Project Details
Project type
Large-Scale EMSL Research
Start Date
2010-10-01
End Date
2013-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members