The affinity and structure of biomolecules immobilized on a 3-D substrate: Time-resolved EPR detection during mobile phase gradients
EMSL Project ID
39731
Abstract
We propose the development of a new magnetic resonance technique to monitor the interactions of biomolecules with binding partners and their environment. Physically, this will be implemented by designing a fritted column to replace the sample holder in the EPR spectrometer. This column will be loaded with biomolecules tethered to a resin substrate. A mobile phase will be flowed through the column and changes in the EPR signal monitored. These changes can reflect a number of different processes, depending on experiment design. For instance, the affinity of the tethered molecule for a paramagnetic species in the mobile phase will be reflected in the increasing EPR signal with time. Similarly, if the tethered molecule has an EPR spectrum that is sensitive to structural changes, the stability of the tethered molecule can be determined by a mobile phase gradient of increasing chaotrope concentration.
Project Details
Start Date
2010-04-01
End Date
2012-11-07
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Dudzik CG, ED Walter, BS Abrams, MS Jurica, and GL Millhauser. 2013. "Coordination of Copper to the Membrane-Bound Form of ?-Synuclein." Biochemistry 52(1):53-60. doi:10.1021/bi301475q