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High Resolution 1H NMR Metabolomics Using Slow and Fast Magic Angle Spinning

EMSL Project ID


We propose to develop both slow and fast magic angle sample spinning NMR methods that is capable of high resolution and high sensitivity metabolic profiling on biological samples, in particular tissue samples, with volume as small as 300 nanoliters (nL) to sample volume as large as a few milliliters. The nL capability will make it possible to follow the metabolic changes through a continued investigation on a single small laboratory animal, and ultimately on a patient, over a long period of time using minimally invasive tissue biopsy and biofluid samples. The large sample size will allow a variety of biological systems with different size to be investigated. These NMR metabolic profiling methods, including the standard solution NMR metabolomics, will be/are being extensively used by us for the studying of disease progression in obesity, atherosclerosis (i.e., the fat accumulation in the part of the artery that is directly linked to the heart), and the impact of low dose radiation to the metabolic profile using mouse models.

Project Details

Project type
Exploratory Research
Start Date
End Date


Principal Investigator

Jian-zhi Hu
Pacific Northwest National Laboratory

Team Members

Ruth Globus
NASA Ames Research Center

Ju Feng
Pacific Northwest National Laboratory

Jason Skouson
Pacific Northwest National Laboratory

Donald Rommereim
Environmental Molecular Sciences Laboratory

Related Publications

Feng J, JZ Hu, SD Burton, and DW Hoyt. 2013. "High Resolution Magic Angle Spinning 1H-NMR Metabolic Profiling of Nanoliter Biological Tissues at High Magnetic Field ." Chinese Journal of Magnetic Resonance 30(1):1-11.
Feng J, NG Isern, SD Burton, and JZ Hu. 2013. "Studies of Secondary Melanoma on C57BL/6J Mouse Liver Using 1H NMR Metabolomics." Metabolites 3:1011-1035. doi:10.3390/metabo3041011
Kottman RM, AA Kulkarni, KA Smolnycki, E Lyda, T Dahanayake, R Salibi, S Honnons, C Jones, NG Isern, JZ Hu, SD Nathan, G Grant, RP Phipps, and PJ Sime. 2012. "Lactic Acid is Elevated in Idiopathic Pulmonary Fibrosis and Induces Myofibroblast Differentiation Via pH-Dependent Activation of Transforming Growth Factor-?." American Journal of Respiratory and Critical Care Medicine 186(8):740-751. doi:10.1164/rccm.201201-0084OC