Microfluidic Devices for Improving Protein Identification and Reducing the Sample Sizes Required for Proteomic Analyses
EMSL Project ID
42691
Abstract
We propose to utilize the EMSL microfabrication facility to create microfluidic devices for improving proteomics analyses. Integrated function microchips will be developed that handle the necessary sample processing steps for preparing biological samples (e.g., individual cells) that are too small to be prepared using existing methods. Additionally, microfluidic capillary electrophoresis devices will couple with capillary liquid chromatography (LC) separations to dramatically increase the peak capacity of the overall separation, and post-LC sample treatment on microfluidic devices will be employed to overcome the limitations of existing protein- or peptide-based proteomic analyses. The newly recapitalized EMSL microfabrication facility will provide state-of-the-art tools for development of these new microfluidic devices.
Project Details
Start Date
2010-11-09
End Date
2011-11-13
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Sun X, RT Kelly, K Tang, and RD Smith. 2011. "Membrane-Based Emitter for Coupling Microfluidics with Ultrasensitive Nanoelectrospray Ionization-Mass Spectrometry." Analytical Chemistry 83(4):5797-5803. doi:10.1021/ac200960h