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Microfluidic Devices for Improving Protein Identification and Reducing the Sample Sizes Required for Proteomic Analyses


EMSL Project ID
42691

Abstract

We propose to utilize the EMSL microfabrication facility to create microfluidic devices for improving proteomics analyses. Integrated function microchips will be developed that handle the necessary sample processing steps for preparing biological samples (e.g., individual cells) that are too small to be prepared using existing methods. Additionally, microfluidic capillary electrophoresis devices will couple with capillary liquid chromatography (LC) separations to dramatically increase the peak capacity of the overall separation, and post-LC sample treatment on microfluidic devices will be employed to overcome the limitations of existing protein- or peptide-based proteomic analyses. The newly recapitalized EMSL microfabrication facility will provide state-of-the-art tools for development of these new microfluidic devices.

Project Details

Start Date
2010-11-09
End Date
2011-11-13
Status
Closed

Team

Principal Investigator

Ryan Kelly
Institution
Brigham Young University

Team Members

Nitin Agrawal
Institution
George Mason University

Xuefei Sun
Institution
Pacific Northwest National Laboratory

Related Publications

Sun X, RT Kelly, K Tang, and RD Smith. 2011. "Membrane-Based Emitter for Coupling Microfluidics with Ultrasensitive Nanoelectrospray Ionization-Mass Spectrometry." Analytical Chemistry 83(4):5797-5803. doi:10.1021/ac200960h