Nanoparticle design, synthesis and characterization for NIH Center Integrating Structure, Activity, Biokinetics and Response for Engineered Nanoparticle (ENP) Risk Assessment
EMSL Project ID
44635
Abstract
This work provides the essential materials science base for designing, synthesizing and delivering three types of nanoparticles for three mechanistic nanotoxicology related biological studies being conducted as part of an NIH center program at PNNL that is also linked to five other centers located around the country. The Material Science Core of the NIH program is organized into three main components: 1) manufacture of primary particles (amorphous silica, cerium, and iron oxides) with specific surface functionality; 2) the modification of the primary particles by addition of fluorescent labels to support tracking and quantification in the biological systems under study, and; 3) characterization of nanoparticles including the kinetics of changes in different media. One major focus of the Material Science Core is completing characterizations in the most appropriate environment for each use- of aerosols for inhalation studies, in cell culture media for in vitro studies, and in vivo tissue samples where called for in animal studies. EMSL capabilities are primarily to be used as an essential component of the particle characterization. A key strength of the Material Science Core is the intent and ability to follow material property transformations in time, providing for characterization of the dynamic nature of ENP characteristics and assuring that these dynamics are not significant confounders to the biological studies. The most important outcomes of work conducted under the Material Science Core will be consistent design and delivery of materials in the appropriate systems, providing for strong Quantitative Structure Activity Relationship (QSAR) modeling and the ability to compare results against results for other particles within our Program and within the consortium. Output of the research will be publications in materials and biological areas. This research on nanoparticle interactions with biological systems directly matches one element of the EMSL 2011 Science of Interfacial Phenomena portion of the Science Theme call for proposals.
Project Details
Project type
Large-Scale EMSL Research
Start Date
2011-10-01
End Date
2014-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Baer DR. 2011. "Surface Characterization of Nanoparticles: Critical Needs and Significant Challenges." Journal of Surface Analysis 17(3):163-169.
Baer D.R. 2020. "Guide to Making XPS Measurements on Nanoparticles." Journal of Vacuum Science and Technology A--Vacuum, Surfaces and Films 38, no. 3:Article No. 031201. PNNL-SA-149921. doi:10.1116/1.5141419
Baer DR, AS Karakoti, P Munusamy, BD Thrall, JG Pounds, JG Teeguarden, JE Amonette, G Orr, PG Tratnyek, and JT Nurmi. 2011. "Testing in EHS: What is the Current Status of Experimentation?" Presented by Don Baer (Invited Speaker) at IEEE Nano 2011, Portland, OR on August 16, 2011. PNNL-SA-83764.
Baer DR, MH Engelhard, GE Johnson, J Laskin, J Lai, KT Mueller, P Munusamy, S Thevuthasan, H Wang, NM Washton, AC Elder, BL Baisch, AS Karakoti, SVNT Kuchibhatla, and DW Moon. 2013. "Surface Characterization of Nanomaterials and Nanoparticles: important needs and challenging opportunities." Journal of Vacuum Science and Technology A--Vacuum, Surfaces and Films 31(5):Article No. 050820. doi:10.1116/1.4818423.
Cartwright MM, SC Schmuck, C Corredor, BB Wang, DK Scoville, C Chisholm, HW Wilkerson, Z Afsharinejad, T Bammler, JD Posner, V Shutthanandan, DR Baer, S Mitra, WA Altemeier, and TJ Kavanagh. 2016. "The pulmonary inflammatory response to multiwalled carbon nanotubes is influenced by gender and glutathione synthesis." Redox Biology 9:264-275. doi:10.1016/j.redox.2016.08.009
Engelhard MH, JN Smith, and DR Baer. 2016. "Gold/silver Core-shell 20 nm Nanoparticles Extracted from Citrate Solution Examined by XPS." Surface Science Spectra 23(1):Article No. 29. doi:10.1116/1.4953792
Karakoti AS, P Munusamy, KE Hostetler, VK Kodali, SVNT Kuchibhatla, G Orr, JG Pounds, JG Teeguarden, BD Thrall, and DR Baer. 2012. "Preparation and Characterization Challenges to Understanding Environmental and Biological Impacts of Nanoparticles." Surface and Interface Analysis 44(8):881-889. doi:10.1002/sia.5006.
Kuchibhatla S.V., A.S. Karakoti, A.E. Vasdekis, C.F. Windisch, S. Seal, S. Thevuthasan, and D.R. Baer. 2019. "An unexpected phase transformation of ceria nanoparticles in aqueous media." Journal of Materials Research 34, no. 3:465-473. PNNL-SA-137435. doi:10.1557/jmr.2018.490
Kuchibhatla SVNT, AS Karakoti, DR Baer, S Samudrala, MH Engelhard, JE Amonette, S Thevuthasan, and S Seal. 2012. "Influence of Aging and Environment on Nanoparticle Chemistry – Implication to Confinement Effects in Nanoceria." Physical Chemistry Chemical Physics. PCCP 116(26):14108-14114. doi:dx.doi.org/10.1021/jp300725s
Kuchibhatla SVNT, AS Karakoti, S Thevuthasan, S Seal, and DR Baer. 2011. "Influence of Aging on the Properties of Cerium Oxide Nanoparticles Implications to Quantum Confinement Effect." In 11th IEEE conference on Nanotchenology 2011. PNNL-SA-81210, Pacific Northwest National Laboratory, Richland, WA.
Munusamy P, CM Wang, MH Engelhard, DR Baer, JN Smith, C Liu, VK Kodali, BD Thrall, S Chen, PE Alexandra, and MP Ryan. 2015. "Comparison of 20 nm silver nanoparticles synthesized with and without a gold core: Structure, dissolution in cell culture media, and biological impact on macrophages." Biointerphases 10(3):031003-1 to 031003-16. doi:10.1116/1.4926547
Sharma G, VK Kodali, MJ Gaffrey, W Wang, KR Minard, NJ Karin, JG Teeguarden, and BD Thrall. 2013. "Iron Oxide Nanoparticle Agglomeration Influences Dose-Rates and Modulates Oxidative Stress Mediated Dose-Response Profiles In Vitro." Nanotoxicology 8(6):663-675. doi:10.3109/17435390.2013.822115