Characterization of the affects of specialized acyl carrier proteins on unusual LPS-hydroxy fatty acids in Geobacter metallireducens
EMSL Project ID
44700
Abstract
Many bacteria have specialized acyl carrier proteins (ACPs) that are involved in synthesis of diverse lipopolysaccharides (LPS). Diversity within LPS aids in survival of Gram-negative bacteria in various environments and is one of the major virulence factors in Gram-negative pathogens. Very little is known about the altered acylation patterns of LPS due to specialized ACPs. In order to determine the effects of the specialized ACPs on LPS-hydroxy fatty acids, (OH-FAs) in G. metallireducens, we will use mass spectrometry to characterize the LPS OH-FAs after knockout of the two specialized ACPs, GmACP2 and GmACP3. Preliminary work will be done on G. metallireducens that have been transformed with plasmids for overexpression of these proteins. In addition, NMR characterization of apo- and holo- ACPs will be used to better understand the relationship between the structure and dynamics of these proteins and their ability to recognize the specific set of proteins involved in LPS modification.
Project Details
Project type
Large-Scale EMSL Research
Start Date
2011-10-01
End Date
2014-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Ramelot TA, PM Rossi, F Forouhar, HW Lee, Y Yang, S Ni, S Unser, S Lew, J Seetharaman, R Xiao, T Acton, JK Everett, JH Prestegard, JF Hunt, G Montelione, and MA Kennedy. 2012. "Structure of a Specialized Acyl Carrier Protein Essential for Lipid A Biosynthesis with Very Long-chain Fatty Acids in Open and Closed Conformations." Biochemistry 51(37):7239-7249. doi:10.1021/bi300546b