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NMR-based metabolomics of the enteropathogens Salmonella and Yersinia


EMSL Project ID
44753

Abstract

The goal of this research is to analyze and reconstruct intercellular and intracellular networks in Salmonella and Yersinia using multiple types of -omics data. Although we hope to gain a more complete understanding of bacterial infection and pathogenicity, the results of this research have wider implications related to the EMSL mission and the needs of DOE, as these are also important pathogens in biodefense. The EMSL LC-NMR with metabolomics cryoprobe is essential for this research as it operates in a high-throughput mode enabling the characterization of metabolites in the large number of samples generated for these studies. EMSL also has a license for the most up-to-date version of Chenomx, a suite of tools that greatly increases the speed at which metabolites can be identified in biological samples. Access to the suite of high-field spectrometers for protein structure analysis allows this complementary research to be done in parallel with metabolomic and proteomic analyses.

Project Details

Project type
Large-Scale EMSL Research
Start Date
2011-10-01
End Date
2013-09-30
Status
Closed

Team

Principal Investigator

Kathleen McAteer
Institution
Washington State University Tri-Cities

Team Members

Carrie Marean-Reardon
Institution
Oregon State University

Athena Metaxas
Institution
University of Texas at Austin

Ernesto Nakayasu
Institution
Pacific Northwest National Laboratory

Charles Ansong
Institution
National Institutes of Health

Vladimir Motin
Institution
University of Texas Medical Branch

Bernhard Palsson
Institution
University of California, San Diego

Thomas Metz
Institution
Pacific Northwest National Laboratory

Fred Heffron
Institution
Oregon Health & Science University

Joshua Adkins
Institution
Pacific Northwest National Laboratory

John Cort
Institution
Pacific Northwest National Laboratory

Related Publications

Charusanti P, S Chauhan, K Mcateer, JA Lerman, DR Hyduke, VL Motin, C Ansong, JN Adkins, and BO Palsson. 2011. "An Experimentally-Supported Genome-Scale Metabolic Network Reconstruction for Yersinia pestis CO92." BMC Systems Biology 5(1):163. doi:10.1186/1752-0509-5-163