NMR-based metabolomics of the enteropathogens Salmonella and Yersinia
EMSL Project ID
44753
Abstract
The goal of this research is to analyze and reconstruct intercellular and intracellular networks in Salmonella and Yersinia using multiple types of -omics data. Although we hope to gain a more complete understanding of bacterial infection and pathogenicity, the results of this research have wider implications related to the EMSL mission and the needs of DOE, as these are also important pathogens in biodefense. The EMSL LC-NMR with metabolomics cryoprobe is essential for this research as it operates in a high-throughput mode enabling the characterization of metabolites in the large number of samples generated for these studies. EMSL also has a license for the most up-to-date version of Chenomx, a suite of tools that greatly increases the speed at which metabolites can be identified in biological samples. Access to the suite of high-field spectrometers for protein structure analysis allows this complementary research to be done in parallel with metabolomic and proteomic analyses.
Project Details
Project type
Large-Scale EMSL Research
Start Date
2011-10-01
End Date
2013-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Charusanti P, S Chauhan, K Mcateer, JA Lerman, DR Hyduke, VL Motin, C Ansong, JN Adkins, and BO Palsson. 2011. "An Experimentally-Supported Genome-Scale Metabolic Network Reconstruction for Yersinia pestis CO92." BMC Systems Biology 5(1):163. doi:10.1186/1752-0509-5-163