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Novel flow based biomolecule immobilization on nanoporous scaffolds
for enhanced biosensor performance and drug delivery applications

EMSL Project ID
44893
Award DOI
https://dx.doi.org/10.46936/genr.proj.2011.44893/60004832

Abstract

 Biosensors play indispensible roles in disease diagnosis, drug screening, and forensic applications. One important area of biosensor research is the immobilization of biomolecules (i.e. enzyme, DNA) with retained or enhanced activities as it is critical to enhance biosensor performance. Nanoporous scaffolds hold an enormous potential in improving the performance of biosensors as porous scaffolds can serve as excellent encapsulating matrix to various bioagents. The central objective of this PNNL proposal is to use the knowledge base gained recently by PI's group on synthesizing biocompatible and stable nanoporous scaffolds with microfluidics method, to develop a robust biomolecule immobilization procedure for enhanced biosensor performances and drug delivery applications. Specific research objectives
are: (1) Tuning the morphology of flow induced nanoporous scaffolds by varying flow conditions via different microfluidic designs with both experiments and detailed nano-morphology studies; (2) Optimization of biomolecule immobilization in nanoporous scaffolds for sensor applications, including enzyme and DNA encapsulation with sensor performance tests.
This proposed research would lead to the discovery of novel scaffolds and biomolecule immobilization methods, that open up new pathways for designing simple, fast, biocompatible, and cost-effective process for enhanced sensor performance and on-site testing
of a variety of biomolecules.

Project Details

Project type
Exploratory Research
Start Date
2011-06-24
End Date
2012-06-24
Status
Closed
Released Data Link
https://search.emsl.pnnl.gov/?project_id_search=44893

Team

Principal Investigator

Amy Shen
Institution
University of Washington

Team Members

Perry Cheung
Institution
University of Washington

Donglai Lu
Institution
Pacific Northwest National Laboratory

Alice Dohnalkova
Institution
Environmental Molecular Sciences Laboratory

Related Publications

Cardiel Rivera JJ, A Dohnalkova, N Dubash, Y Zhao, P Cheung, and A Shen. 2013. "Microstructure and Rheology of a Flow-Induced Structured Phase in Wormlike Micellar Solutions." Proceedings of the National Academy of Sciences of the United States of America 110(18):E1653-E1660. doi:10.1073/pnas.1215353110
Cardiel Rivera JJ, L Tonggu, A Dohnalkova, P de la Iglesia, DC Pozzo, and A Shen. 2013. "Worming Their Way into Shape: Toroidal Formations in Micellar Solutions." ACS Nano 7(11):9704-9713. doi:10.1021/nn404191s