Determination of the protein composition of a novel membrane bound pigment protein complex involved in solar energy capture
EMSL Project ID
45192
Abstract
Photosynthetic organisms such as cyanobacteria can efficiently convert sunlight into usable forms of energy. These organisms use pigment protein antenna systems to capture light and transfer it to reaction centers where photochemistry occurs. Cyanobacteria can modify their antenna systems in response to changing environmental conditions. In one important environmental response, when stressed for iron, an additional antenna ring complex around photosystem I (PSI) is formed by the IsiA protein. However, many details of IsiA function are not well understood. In particular, the mechanism of ring formation has not been determined and any proteins involved in the assembly of the complex have not been identified. In order to more completely characterize IsiA-containing complexes, we have generated a Synechocystis sp. PCC 6803 strain with a C-terminal histidine tag on IsiA. Complexes isolated by this approach appear to contain PSI and IsiA, but the identity of other proteins that comprise the complex is unknown. Here we propose to identify the entire pool of proteins that associate with IsiA and assemble into ring-shaped complexes. The facilities at EMSL are ideal for such studies, and we have a history of successfully working with EMSL on related proteomics projects. This work is important to fully understand the role of IsiA in energy capture and response to environmental stress in cyanobacteria.Project Details
Project type
Limited Scope
Start Date
2011-08-15
End Date
2011-10-15
Status
Closed
Released Data Link
Team
Principal Investigator