Systematic Characterization of Protein Glycosylation of Bacteria Cell Surface Proteins
EMSL Project ID
45394
Abstract
The overall goal of this proposal is to develop an integrated top-down and bottom-up approach for bacterial glycoprotein profiling assisted by accurate glycan structure identification, which will be applied to study bacterial glycosylations. This platform will combine multiple cutting-edge "omics" techniques (proteomics, glycomics, and bioinformatics) uniquely provided by EMSL: (1) state-of-the-art instrumentation (FTMS, including high field FTICR MS, high resolution NMR); (2) versatile in-house built high performance LC platforms, which provide unparalleled throughput and sensitivity for both intact proteins and peptides; (3) computing capabilities and in-house software developments; and (4) staff expertise in the areas of MS, HPLC, NMR and bioinformatics.Using the proposed platform, we will test the hypothesis that the structure of the glycan moieties of thermophilic bacteria glycoproteins are different from those of bacteria that thrive at low temperatures. To accomplish this, we will perform the following tasks:
1. Apply our proposed platform to profile the glycoproteome in Synechococcus sp. strain PCC 7002, a model cyanobacteria system for which limited information on glycosylations is already available.;
2. Apply our proposed platform to find the glycoproteome signature in extremely thermophilic bacteria, T. maritima and K. olearia.
Project Details
Start Date
2011-07-11
End Date
2014-07-20
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members