Rapid Access Proposal: Metaproteomics method optimization for soil and sediments
EMSL Project ID
45997
Abstract
The Jansson lab at LBNL has several DOE and other funded projects that aim to gain a better understanding of microbial community function in soils and sediments. The projects have high DOE relevance and include focus on understanding how microorganisms cycle carbon (important for understanding impact of climate change) and degrade pollutants. The lab has prior expertise in the area of environmental proteomics through collaborations with scientists at ORNL (Robert Hettich and colleagues), including recent publication of a method for extraction of proteins from soil (Chourey et al., 2010). Currently, the Jansson laboratory has several new projects that would benefit from metaproteomics analysis, but the sample matrices are difficult and some have a low biomass. The plan is to submit a full proposal to EMSL for analyses of these samples. However, first it would be advantageous to confirm that the protein extraction methods and analysis pipelines work on these sample sets.
Project Details
Project type
Limited Scope
Start Date
2012-01-01
End Date
2012-03-02
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members