Urinary Proteome Monitoring for Transplant Injury
EMSL Project ID
46197
Abstract
We propose to achieve the objective of identifying novel urinary biomarkers for selected categories of renal transplant injury in two phases. We will first employ a discovery phase to comprehensively scan the urinary proteome by high-throughput proteomics, utilizing cross-sectional urine samples banked from renal transplant patients at Stanford University. In the second pre-clinical validation phase a large cohort of serially collected, banked urine samples at Stanford University from a multicenter prospective clinical trial, will be used to validate the biomarker proteins by high throughput LC-SRM-MS. The deliverable is a highly selected and validated set of urinary proteins and peptides that can be used to develop new assays or tests for serial, clinical monitoring patient urine samples in an out-patient setting, specific and sensitive for categories of graft injury relating to acute rejection (AR), chronic allograft nephropathy (CAN), stable transplants without significant injury (STA) and BK viremia/vyuria (BKV).
Project Details
Start Date
2012-01-23
End Date
2014-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Sigdel TK, N Salomonis, CD Nicora, S Ryu, J He, V Dinh, DJ Orton, RJ Moore, SC Hsieh, H Dai, TV Mindh, W Xiao, RD Smith, W Qian, DG Camp, II, and MM Sarwal. 2014. "The Identification of Novel Potential Injury Mechanisms and Candidate Biomarkers in Renal Allograft Rejection by Quantitative Proteomics." Molecular & Cellular Proteomics. MCP 13(2):621-31. doi:10.1074/mcp.M113.030577
Sigdel TK, N Salomonis, CD Nicora, S Ryu, J He, V Dinh, DJ Orton, RJ Moore, SC Hsieh, H Dai, TV Mindh, W Xiao, RD Smith, W Qian, DG Camp, II, and MM Sarwal. 2014. "The Identification of Novel Potential Injury Mechanisms and Candidate Biomarkers in Renal Allograft Rejection by Quantitative Proteomics." Molecular & Cellular Proteomics. MCP 13(2):621-31. doi:10.1074/mcp.M113.030577
Sigdel TK, Y Gao, J He, A Wang, CD Nicora, TL Fillmore, T Shi, BJM Webb-Robertson, RD Smith, W Qian, O Salvatierra, DG Camp, II, and M Sarwal. 2016. "Mining the Human Urine Proteome for Monitoring Renal Transplant Injury ." Kidney International 89(6):1244-1252. doi:10. 1016/j. kint. 2015. 12. 049
Wu C, J Duan, T Liu, RD Smith, and W Qian. 2016. "Contributions of Immunoaffinity Chromatography to Deep Proteome Profiling of Human Biofluids." Journal of Chromatography B. doi:10.1016/j.jchromb.2016.01.015