Chemical characterization at the cellular level of biological surfaces using cluster probes and high-resolution mass spectrometry
EMSL Project ID
46690
Abstract
We seek to demonstrate the potential uses of cluster probes and high-resolution mass spectrometry for surface interrogation of two specific biological systems. The use of high resolution mass spectrometry for molecular identification is justified due to the high chemical complexity of the biological systems. The first study is focused on the characterization of the chemical composition of brain sections derived from mammalian model systems for various diseases, and their response to drug treatments. An initial set of experiments will center on the analysis of the basal ganglia circuitry under different drug therapies associated with Parkinson-like disease. The second study involves the characterization of single cells at different stages of the cell cycle and different external stimuli (e.g., Dictyostelium discoideum). A single cell model system will be used to study the cellular distribution of fatty acids and lipids as a function of the experimental conditions. In both cases, emphasis will be on the feasibility of using cluster probes coupled to high-resolution mass spectrometry to describe the chemical environment at the cellular level.These results will be used as preliminary results for Dr. Fernandez-Lima's NIH-K99-R00 project renewal (to be submitted in Feb/2012 to NCRR/NIH). These experiments will also provide currently missing chemical information for our preliminary reports to the NIH on Parkinson's disease (NINDS, Feb/2012) and cell motility proposals, coordinated by Dr. Smotherman and Dr. Gomer, respectively. We also anticipate that our experience and interaction with EMSL/PNNL experts will initiate future fruitful collaborations.
Project Details
Project type
Limited Scope
Start Date
2012-05-07
End Date
2012-07-07
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members