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Identification of Functional Proteins Relevant to Bioenergy and Disease Pathology by Multiplexed Activity-Based Protein Profiling


EMSL Project ID
47292

Abstract

Develop multiplexed activity-based protein profiling and apply to identification of P450s in Mycobacterium tuberculosis and biomarkers in Aspergillus fumigatus. Additionally the technology will be used for identifying new sources of bioenergy from botryococcus braunii and extremophilic fungi.

Project Details

Start Date
2012-03-21
End Date
2014-09-30
Status
Closed

Team

Principal Investigator

Aaron Wright
Institution
Pacific Northwest National Laboratory

Team Members

Joshua Rosnow
Institution
Pacific Northwest National Laboratory

Natalie Sadler
Institution
Pacific Northwest National Laboratory

Michael Lewis
Institution
Pacific Northwest National Laboratory

Lindsey Anderson
Institution
Pacific Northwest National Laboratory

Susan Wiedner
Institution
Pacific Northwest National Laboratory

Sergey Stolyar
Institution
University of Idaho

David Culley
Institution
Pacific Northwest National Laboratory

Phillip Koech
Institution
Pacific Northwest National Laboratory

Jon Magnuson
Institution
Pacific Northwest National Laboratory

Ellen Panisko
Institution
Pacific Northwest National Laboratory

Related Publications

Anderson LN, DE Culley, BA Hofstad, LM Chauvign-Hines, EM Zink, SO Purvine, RD Smith, SJ Callister, JM Magnuson, and AT Wright. 2013. "Activity-based protein profiling of secreted cellulolytic enzyme activity dynamics in Trichoderma reesei QM6a, NG14, and RUT-C30." Molecular Biosystems 9(12):2945-3218. doi:10.1039/c3mb70333a
Ansong C, C Ortega, SH Payne, DH Haft, LM Chauvigne-Hines, MP Lewis, SO Purvine, AK Shukla, S Fortuin, RD Smith, JN Adkins, C Grundner, and AT Wright. 2013. "Identification of widespread adenosine nucleotide binding in Mycobacterium tuberculosis." Chemistry & Biology 20(1):123-133. doi:10.1016/j.chembiol.2012.11.008
Bernstein HC, MA Charania, RS McClure, NC Sadler, MR Melnicki, EA Hill, LM Markillie, CD Nicora, AT Wright, MF Romine, and AS Beliaev. 2015. "Multi-omic Dynamics Associate Oxygenic Photosynthesis with Nitrogenase-mediated H2 production in Cyanothece sp. ATCC 51142." Scientific Reports 5:Article No. 16004. doi:10. 1038/srep16004
Liu Y, R Zhang, Z Lian, S Wang, and AT Wright. 2014. "Yeast cell surface display for lipase whole cell catalyst and its applications." Journal of Molecular Catalysis B: Enzymatic 106(August 2014):17-25. doi:10.1016/j.molcatb.2014.04.011
Ortega C, R Liao, LN Anderson, T Rustad, AR Ollodart, AT Wright, DR Sherman, and C Grundner. 2014. "Mycobacterium tuberculosis Ser/Thr protein kinase B mediates an oxygen-dependent replication switch." PLoS Biology 12(1):Article No. e1001746. doi:10.1371/journal.pbio.1001746
Sadler NC, TE Angel, MP Lewis, LM Pederson, LM Chauvigne-Hines, SD Wiedner, EM Zink, RD Smith, and AT Wright. 2012. "Activity-Based Protein Profiling Reveals Mitochondrial Oxidative Enzyme Impairment and Restoration in Diet-Induced Obese Mice." PLoS One 7(10):Article No. e47996. doi:10.1371/journal.pone.0047996
Wright AT, T Magnaldo, RL Sontag, LN Anderson, NC Sadler, PD Piehowski, Y Gache, and TJ Weber. 2013. "Deficient Expression of Aldehyde Dehydrogenase 1A1 Is Consistent with Increased Sensitivity of Gorlin Syndrome Patients to Radiation Carcinogenesis." Molecular Carcinogenesis. doi:10.1002/mc.22115