Genome-Enabled Studies of Photosynthetic Microorganisms for Bioenergy Applications - Chemical Protein Profiling
EMSL Project ID
47297
Abstract
As part of PNNL's Scientific Focus Area "Genome-Enabled Studies of Photosynthetic Microorganisms for Bioenergy Applications" this project is applying and developing click-chemistry enabled probes to develop a predictive understanding of pathways and regulatory schemes involved in solar energy conversion to biofuel precursors or products. To date, knowledge gaps are not limited to biochemical pathways of energy and carbon flux but to an even larger degree include regulatory events within and between metabolic subsystems that include intracellular signals to which transcriptional regulation is responding. We suggest that "redox sensing," as a means to maintain redox homeostasis in photosynthesizing cells, is likely an equally important mechanism. In this project, we are investigating the central hypothesis that these sensors regulate electron flux and carbon partitioning in cyanobacteria. Specifically, we have developed and applied probe approaches for live cell capture, characterization, and imaging of redox sensors of environmental change using advanced separations, mass spectrometry and traditional biochemical techniques.
Project Details
Start Date
2012-09-19
End Date
2014-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Ansong C, NC Sadler, EA Hill, MP Lewis, EM Zink, RD Smith, AS Beliaev, A Konopka, and AT Wright. 2014. "Characterization of protein redox dynamics induced during light-to-dark transitions and nutrient limitation in cyanobacteria." Frontiers in Microbiology 5(325):, doi:10.3389/fmicb.2014.00325
Beliaev AS, M Serres, BE Linggi, LM Markillie, NG Isern, WB Chrisler, LA Kucek, EA Hill, G Pinchuk, DA Bryant, HS Wiley, MF Romine, JK Fredrickson, and A Konopka. 2013. "INTERACTIONS BETWEEN CYANOBACTERIA AND HETEROTROPHS UNDER DIFFERENT TROPHIC CONDITIONS." PNNL-SA-98626, Pacific Northwest National Laboratory, Richland, WA. In review.
Sadler NC, MR Melnicki, MH Serres, ED Merkley, WB Chrisler, EA Hill, MF Romine, S Kim, EM Zink, S Datta, RD Smith, AS Beliaev, A Konopka, and AT Wright. 2014. "Live Cell Chemical Profiling of Temporal Redox Dynamics in a Photoautotrophic Cyanobacterium." ACS Chemical Biology [Epub Ahead of Print]:, doi:10.1021/cb400769v