A portable multimodal microfluidic flow cell for in situ imaging of liquid by ToF-SIMS and SEM
EMSL Project ID
47299
Abstract
Our technical and scientific objective is to provide a universal portable multimodal flow cell for in situ studies of dynamic liquid surfaces in various applications. Our business goal is to make the microfluidic platform suitable for developing into a new product for use with more than one analytical tool, namely, time-of-flight secondary ion mass spectrometry (ToF-SIMS) and scanning electron microscopy (SEM) by collaborating with our industrial partners SPI Supplies (SPI) and ION-TOF USA, Inc. (ION-TOF). Studying liquid samples such as biological cells in situ in their native liquid environment presents a significant technical challenge, because it requires working with volatile liquids in vacuum and introducing samples into a device without damage. The experimental design and methods are based on our recent invention which enables in situ observations of liquid surfaces using ToF-SIMS and SEM via a vacuum compatible interface made of a novel microfluidic flow cell. In this project, we will aim at hardening the device by 1) providing quantitative characterizations using ToF-SIMS and SEM, and 2) illustrating the feasibility of applying it in diverse environments including observations of solid particles in a dynamic flow, biological samples in aqueous environment, intermediate species in electrochemical reactions, and nanoparticles suspended in solid-liquid interface. The development and commercialization of the portable microfluidic module enables multimodal studies of various samples in liquids for the first time. It will open a new product line that allows multifaceted analyses in various applications in the near future.
Project Details
Start Date
2012-02-27
End Date
2013-03-10
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Hua X, MJ Marshall, Y Xiong, X Ma, Y Zhou, AE Tucker, Z Zhu, S Liu, and XY Yu. 2015. "Two-dimensional and three-dimensional dynamic imaging of live biofilms in a microchannel by time-of-flight secondary ion mass spectrometry." Biomicrofluidics 9(3):Article No. 031101. doi:10.1063/1.4919807
Hua X, XY Yu, Z Wang, L Yang, B Liu, Z Zhu, AE Tucker, WB Chrisler, EA Hill, S Thevuthasan, Y Lin, S Liu, and MJ Marshall. 2014. "In situ molecular imaging of hydrated biofilm in a microfluidic reactor by ToF-SIMS." Analyst 139:1609-1613. doi:10.1039/C3AN02262E
Liu B, D Du, X Hua, XY Yu, and Y Lin. 2014. "Papers Based Electrochemical Biosensors: From Test Strips to Paper-Based Microfluidics." Electroanalysis 26(6):1214-1223. doi:10.1002/elan.201400036
Liu B, XY Yu, Z Zhu, X Hua, L Yang, and Z Wang. 2014. "In situ chemical probing of the electrode-electrolyte interface by ToF-SIMS." Lab on a Chip 14:855-859. doi:10.1039/C3LC50971K
Shard AG, R Havelund, SJ Spencer, IS Gilmore, MR Alexander, TB Angerer, S Aoyagi, J-P Barnes, A Benayad, A Bernasik, G Ceccone, JDP Counsell, C Deeks, JS Fletcher, DJ Graham, C Heuser, TG Lee, C Marie, MM Marzec, G Mishra, D Rading, O Renault, DJ Scurr, HK Shon, V Spampinato, H Tian, F Wang, N Winograd, K Wu, A Wucher, Y Zhou, and Z Zhu. 2015. "Measuring Compositions in Organic Depth Profiling: Results from a VAMAS Interlaboratory Study." The Journal of Physical Chemistry B. doi:10.1021/acs.jpcb.5b05625
Wang Z, Y Zhang, B Liu, K Wu, S Thevuthasan, DR Baer, Z Zhu, XY Yu, and F Wang. 2017. "In Situ Mass Spectrometric Monitoring Of The Dynamic Electrochemical Process At The Electrode-Electrolyte Interface: A SIMS Approach." Analytical Chemistry 89(1):960-965. doi:10.1021/acs.analchem.6b04189