Validation and Advanced Development of Emerging Technologies
EMSL Project ID
47301
Abstract
Develop mass spectrometry (MS) based assays for quantitative biomarker validation with sensitivity and specifically comparable to or better than antibody based immunoassays. The new liquid chromatography-electrospray ionization-multiple reaction monitoring MS instrument will be applicable to a broad spectrum of clinical cancer biomarker validation applications.
Project Details
Start Date
2012-03-21
End Date
2014-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Cox JT, I Marginean, RD Smith, and K Tang. 2014. "On the Ionization and Ion Transmission Efficiencies of Different ESI-MS Interfaces." Journal of the American Society for Mass Spectrometry . doi:10.1007/s13361-014-0998-5 [In Press]
Cox JT, I Marginean, RT Kelly, RD Smith, and K Tang. 2014. "Improving the Sensitivity of Mass Spectrometry by Using a New Sheath Flow Electrospray Emitter Array at Subambient Pressures ." Journal of the American Society for Mass Spectrometry Epub Ahead of Print:, doi:10.1007/s13361-014-0856-5
Cox JT, SR Kronewitter, AK Shukla, RJ Moore, RD Smith, and K Tang. 2014. "High Sensitivity Combined with Extended Structural Coverage of Labile Compounds via Nanoelectrospray Ionization at Subambient Pressures." Analytical Chemistry 86(19):9504-9511. doi:10.1021/ac502767y
Guo X, TL Fillmore, Y Gao, and K Tang. 2016. "Capillary Electrophoresis-nanoelectrospray Ionization-selected Reaction Monitoring Mass Spectrometry Via A True Sheathless Metal-coated Emitter Interface For Robust And High Sensitivity Sample Quantification." Analytical Chemistry 88(8):4418-4425. doi:10.1021/acs.analchem.5b04912
Li X ,Cox J T,Tang K ,Bieberich C J 2016. "Quantifying Kinase-specific Phosphorylation Stoichiometry Using Stable Isotope Labeling In a Reverse In-gel Kinase Assay" Analytical Chemistry 88():11468-11475. 10.1021/acs.analchem.6b02599
Wang CC, CS Lee, RD Smith, and K Tang. 2013. "Capillary Isotachophoresis-Nanoelectrospray Ionization-Selected Reaction Monitoring MS via a Novel Sheathless Interface for High Sensitivity Sample Quantification." Analytical Chemistry. doi:10.1021/ac401202c
Wang C, CS Lee, RD Smith, and K Tang. 2012. "Ultrasensitive Sample Quantitation via Selected Reaction Monitoring Using CITP/CZE-ESI-Triple Quadrupole MS." Analytical Chemistry 84(23):10395-10403. doi:10.1021/ac302616m