EMSL/FCSD Scientific Partner Proposal on the Dynamics of Biological and Catalytic Systems
EMSL Project ID
47430
Abstract
The goal of this Scientific Partner Proposal is to develop the capability to observe “live” biological and catalytic processes in the dynamic transmission electron microscope (DTEM). Live biological processes have previously only been observed by light microscopy at low spatial resolution (~200nm), while the molecular details of biological samples have only been studied at high-resolution (~1 nm) in the arrested (fixed/frozen) state by cryo-electron microscopy (cryo-EM). Here we propose to merge these capabilities by assembling a DTEM on the basis of a double-corrected TEM with in-column energy filter. The final instrument will have the capability to record structural and chemical information in real-time with sub-molecular resolution. To benchmark these observations appropriately, this partnership will also install and operate a 300kV liquid-He cryo-EM. Both instruments will be provided by FCSD and installed in the new EMSL Q-wing (providing the best environmental conditions) and will involve the expertise of scientists in both EMSL and FCSD to realize the full potential of these microscopes. Together these two microscopes will provide unprecedented imaging capabilities to observe biological interfaces, interactions and dynamics and operand catalytic processes.
Project Details
Project type
Scientific Partner
Start Date
2013-07-12
End Date
2014-10-21
Status
Closed
Released Data Link
Team
Principal Investigator
Co-Investigator(s)
Team Members
Related Publications
Cole JK, JR Hutchison, RS Renslow, YM Kim, WB Chrisler, HE Engelmann, A Dohnalkova, D Hu, TO Metz, JK Fredrickson, and SR Lindemann. 2014. "Phototrophic Biofilm Assembly in Microbial-Mat-Derived Unicyanobacterial Consortia: Model Systems for the Study of Autotroph-Heterotroph Interactions." Frontiers in Microbiology 5:Article No. 109. doi:10.3389/fmicb.2014.00109