Biomarkers for pulmonary injury following deployment
EMSL Project ID
47697
Abstract
This project is designed to measure the physiological response of deployment using small RNA and the global protein composition levels in blood serum, urine, and broncho-alveolar lavage fluid (BALF) as the measure of that response. Samples were obtained from volunteer soldiers by Dr. Matthew Morris at MD Anderson and will be sent to the ISB for aliquoting for analysis of small RNAs and then to PNNL for global protein composition for serum, urine, and BALF samples. The samples will be selected for proteome analysis as determined based on collaborative scientific input by Dr. Gelinas and Dr. Jackson with resource constraints. The underlying hypothesis is that the proteome in serum and BALF may provide insights to the physiological response to deployment. Hence, this sample selection process is particularly important for attaining project goals because certain time points/samples may be critically important for analysis while others may be largely uninformative. Once, the appropriate samples are selected for analysis that maximize the experimental information content with the project's budget, PNNL will perform depletion of abundant proteins from serum and/or BALF as based on commercially available immune-affinity depletion columns. Following depletion, the depleted plasma/BALF will be subject to mass spectrometry-based proteomics analysis. Proteomics will be performed to using liquid chromatography coupled high resolution coupled mass spectrometry using platforms developed at PNNL using commercial and in-house developed components. Once the proteomics data is acquired is will be processed by both commercial and PNNL-developed informatics software to infer and quantify the proteins present in the serum and/or BALF samples. Experiments will be batched and randomized to avoid any time or order effects in the LC-MS/MS process. Interpretation of the proteomics results will be primarily driven by PNNL in collaboration with Drs. Gelinas and Jackson. Overall interpretation will be done collaboratively as focused by programmatic expectations and empirical experimental results.
Project Details
Start Date
2012-10-04
End Date
2013-10-10
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members