Optimizing Oil Production in Oleaginous Yeast by Cell-Wide Measurements and Genome-Based Models
EMSL Project ID
47736
Abstract
Develop tools for cell-wide measurement of metabolites and lipids, which, along with transcriptional data, will allow the construction of genome-scale metabolic models, as well as models of transcriptional regulation, that will guide the further metabolic engineering of Y. lipolytica. This engineering will aim at improving the following fermentation characteristics of the present strain, all important figures of merit in the development of a cost-effective process for the conversion of renewable resources to lipids for biofuel production:• Improve conversion yield of glucose to greater than 0.25 grams of oil per gram of glucose consumed
• Improve total volumetric productivities to double of present values
• Facilitate utilization of acetate as substrate for lipid production and increase the overall conversion yield to lipids, and,
• Improve xylose assimilation and conversion to lipids.
Project Details
Start Date
2012-11-21
End Date
2013-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Pomraning KR, S Wei, SA Karagiosis, YM Kim, A Dohnalkova, BW Arey, EL Bredeweg, G Orr, TO Metz, and SE Baker. 2015. "Comprehensive metabolomic, lipidomic and microscopic profiling of Yarrowia lipolytica during lipid accumulation identifies targets for increased lipogenesis." PLoS One 10(4):e0123188. doi:10.1371/journal.pone.0123188