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Optimizing Oil Production in Oleaginous Yeast by Cell-Wide Measurements and Genome-Based Models


EMSL Project ID
47736

Abstract

Develop tools for cell-wide measurement of metabolites and lipids, which, along with transcriptional data, will allow the construction of genome-scale metabolic models, as well as models of transcriptional regulation, that will guide the further metabolic engineering of Y. lipolytica. This engineering will aim at improving the following fermentation characteristics of the present strain, all important figures of merit in the development of a cost-effective process for the conversion of renewable resources to lipids for biofuel production:
• Improve conversion yield of glucose to greater than 0.25 grams of oil per gram of glucose consumed
• Improve total volumetric productivities to double of present values
• Facilitate utilization of acetate as substrate for lipid production and increase the overall conversion yield to lipids, and,
• Improve xylose assimilation and conversion to lipids.

Project Details

Start Date
2012-11-21
End Date
2013-09-30
Status
Closed

Team

Principal Investigator

Scott Baker
Institution
Environmental Molecular Sciences Laboratory

Team Members

David Culley
Institution
Pacific Northwest National Laboratory

Thomas Metz
Institution
Pacific Northwest National Laboratory

Related Publications

Pomraning KR, S Wei, SA Karagiosis, YM Kim, A Dohnalkova, BW Arey, EL Bredeweg, G Orr, TO Metz, and SE Baker. 2015. "Comprehensive metabolomic, lipidomic and microscopic profiling of Yarrowia lipolytica during lipid accumulation identifies targets for increased lipogenesis." PLoS One 10(4):e0123188. doi:10.1371/journal.pone.0123188