Secretomes from soil fungi in temperate forests and arid grasslands that are responsive to altered C and N deposition
EMSL Project ID
47926
Abstract
Temperate ecosystems are experiencing increasing levels of atmospheric CO2 and nitrogen (N) deposition, which alter plant productivity and the amount and quality of above and belowground carbon. The composition and activity of soil fungi that decompose dead plant tissue will likely be impacted by altered plant responses to these agents of global change. Our current research investigates the structure and activities of the fungal community in soils of temperate forests and arid grasslands, in response to long-term alterations in carbon and/or nitrogen inputs. Our use of genomic sequencing, target metagenomics and metatranscriptome approaches, coupled with soil chemical measures, is beginning to identify changes in soil fungal community composition associated with altered C and N inputs. We have identified a selection of fungi representing genera found to be very abundant and/or responsive to altered C or N inputs in a temperate pine forest and an arid grassland. With current support from the Dept. of Energy through a Science Focus Area program, we are isolating these fungi, generating genomic and transcriptomic information from these isolates during growth on a variety of substrates. We propose to broaden the scope of our on-going studies to include a proteomics component to explore the secreted proteome of these fungi growing on different substrates relevant to plant carbon decomposition in soils.
Project Details
Project type
Large-Scale EMSL Research
Start Date
2013-10-01
End Date
2015-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Co-Investigator(s)
Team Members
Related Publications
Challacombe J., J. Challacombe, C.N. Hesse, L.M. Bramer, L. McCue, M.S. Lipton, and S.O. Purvine, et al. 2019. "Genomes and Secretomes of Ascomycota Fungi Reveal Diverse Functions in Plant Biomass Decomposition and Pathogenesis." BMC Genomics 20, no. 1:Article No. 976. PNNL-SA-140986. doi:10.1186/s12864-019-6358-x