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Secretomes from soil fungi in temperate forests and arid grasslands that are responsive to altered C and N deposition


EMSL Project ID
47926

Abstract

Temperate ecosystems are experiencing increasing levels of atmospheric CO2 and nitrogen (N) deposition, which alter plant productivity and the amount and quality of above and belowground carbon. The composition and activity of soil fungi that decompose dead plant tissue will likely be impacted by altered plant responses to these agents of global change. Our current research investigates the structure and activities of the fungal community in soils of temperate forests and arid grasslands, in response to long-term alterations in carbon and/or nitrogen inputs. Our use of genomic sequencing, target metagenomics and metatranscriptome approaches, coupled with soil chemical measures, is beginning to identify changes in soil fungal community composition associated with altered C and N inputs. We have identified a selection of fungi representing genera found to be very abundant and/or responsive to altered C or N inputs in a temperate pine forest and an arid grassland. With current support from the Dept. of Energy through a Science Focus Area program, we are isolating these fungi, generating genomic and transcriptomic information from these isolates during growth on a variety of substrates. We propose to broaden the scope of our on-going studies to include a proteomics component to explore the secreted proteome of these fungi growing on different substrates relevant to plant carbon decomposition in soils.

Project Details

Project type
Large-Scale EMSL Research
Start Date
2013-10-01
End Date
2015-09-30
Status
Closed

Team

Principal Investigator

Cheryl Kuske
Institution
Los Alamos National Laboratory

Co-Investigator(s)

Chris Yeager
Institution
Los Alamos National Laboratory

Team Members

Si Wu
Institution
University of Oklahoma

Related Publications

Challacombe J., J. Challacombe, C.N. Hesse, L.M. Bramer, L. McCue, M.S. Lipton, and S.O. Purvine, et al. 2019. "Genomes and Secretomes of Ascomycota Fungi Reveal Diverse Functions in Plant Biomass Decomposition and Pathogenesis." BMC Genomics 20, no. 1:Article No. 976. PNNL-SA-140986. doi:10.1186/s12864-019-6358-x