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Elucidating the Effects of Gene and Protein Structure on Heterologous Expression and Secretion of Biomass Hydrolyzing Enzymes in Aspergillus niger


EMSL Project ID
47996

Abstract

Filamentous fungi are excellent protein production hosts as evidenced by their wide use in industry to produce a variety of native proteins. However, consistent high titer heterologous protein expression in fungi (or any other class of organisms) remains an elusive yet highly desirable goal. Engineering of the gene or engineering of the production host are two approaches to improving protein expression. We are taking both approaches in our chose production host, Aspergillus niger, and the glycoside hydrolase genes we seek to express from extremophilic bacteria. Proteomics and transcriptomics analysis of samples taken from a culture time course A. niger expressing a heterologous secreted gene will be utilized to identify desirable genes to alter in the host as well as potentially identify sites in the heterologous gene for engineering to obtain better expression.

Project Details

Project type
Large-Scale EMSL Research
Start Date
2013-10-01
End Date
2014-09-30
Status
Closed

Team

Principal Investigator

Jon Magnuson
Institution
Pacific Northwest National Laboratory

Co-Investigator(s)

John Gladden
Institution
Joint BioEnergy Institute